CD19 antibody

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Mouse anti CD19

CD19 antibody recognizes B-lymphocyte antigen CD19, also known as B-lymphocyte surface antigen B4 differentiation antigen CD19 T-cell surface antigen, Leu-12, or CD19. The antibody has been extensively validated for western blotting using whole cell lysates.

CD19 Antibody with Control Lysate

CD19 antibody recognizes B-lymphocyte antigen CD19, also known as B-lymphocyte surface antigen B4 differentiation antigen CD19 T-cell surface antigen, Leu-12, or CD19. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00030 WB 100 µl
VMA00030KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human CD19 antibody recognizes an epitope within the C-terminal cytoplasmic tail sequence of human CD19, a single pass type I transmembrane glycoprotein containing two C2 type Ig-like domains in the N-terminal extracellular region and four potential phosphorylation sites for tyrosine together with a single serine in the cytoplasmic region.

Human CD19 is expressed on virtually all cells of the B-cell lineage with the exception of plasma cells and plays a regulatory role in B-cell differentiation and proliferation. B-cells are essential for antibody production and mutations in the CD19 gene can lead to an immunodeficiency syndrome, CIVD3 characterized by hypogammaglobulinemia leading to recurrent infections and the inability to mount an antibody mediated response to immune insult. Although immunoglobulin production is impaired, B-cell precursors appear in normal numbers together with some reduction in more mature B-cell forms (van Zelm et al. 2006). B-cells have also been implicated in the progression and pathogenesis of multiple sclerosis and are common circulatory components of both active and chronic MS lesions and well as in CSF (Ritchie et al. 2004).

Mouse anti Human CD19 antibody detects a band of ~95 kDa in Western blotting using a Raji B-cell lymphoma cell lysate under reducing conditions.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the C-terminal amino acids CGPDPAWGGGGRMGTWSTR of human CD19 coupled to KLH

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P15391 Related reagents
Entrez Gene
CD19 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti CD19 antibody detects a band of approximately 95 kDa in Raji cell lysates.
Western Blotting
Anti CD19 antibody detects a band of approximately 95 kDa in Raji cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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