CD19 antibody

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Mouse anti CD19

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00030
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human CD19 antibody recognizes an epitope within the C-terminal cytoplasmic tail sequence of human CD19, a single pass type I transmembrane glycoprotein containing two C2 type Ig-like domains in the N-terminal extracellular region and four potential phosphorylation sites for tyrosine together with a single serine in the cytoplasmic region.

Human CD19 is expressed on virtually all cells of the B-cell lineage with the exception of plasma cells and plays a regulatory role in B-cell differentiation and proliferation. B-cells are essential for antibody production and mutations in the CD19 gene can lead to an immunodeficiency syndrome, CIVD3 characterized by hypogammaglobulinemia leading to recurrent infections and the inability to mount an antibody mediated response to immune insult. Although immunoglobulin production is impaired, B-cell precursors appear in normal numbers together with some reduction in more mature B-cell forms (van Zelm et al. 2006). B-cells have also been implicated in the progression and pathogenesis of multiple sclerosis and are common circulatory components of both active and chronic MS lesions and well as in CSF (Ritchie et al. 2004).

Mouse anti Human CD19 antibody detects a band of ~95 kDa in Western blotting using a Raji B-cell lymphoma cell lysate under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti CD19 antibody detects a band of approximately 95 kDa in Raji cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the C-terminal amino acids CGPDPAWGGGGRMGTWSTR of human CD19 coupled to KLH

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P15391 Related reagents
Entrez Gene
CD19 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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