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CD19 antibody | LT19

Mouse anti Human CD19:Pacific Blue®

Product Type
Monoclonal Antibody
Clone
LT19
Isotype
IgG1
Specificity
CD19

Product Code Applications Pack Size List Price Your Price Qty
MCA1940PB
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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MCA1940PBT
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SDS Safety Datasheet SDS
F 25 Tests/0.25ml loader
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Mouse anti Human CD19 antibody, clone LT19 recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rie et al. 1989).

CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21 (Bradbury et al. 1992), CD9, CD81 and CD82 (Horváth et al. 1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzutto et al. 1987).

Target Species
Human
Product Form
Purified IgG conjugated to Pacific Blue® - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Pacific Blue® 410 455
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Pacific Blue® MCA928PB F 100 Tests/1ml loader
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List Price Your Price
loader
Description Mouse IgG1 Negative Control:Pacific Blue®

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD19 antibody

  1. Hughes, G.J. et al. (2007) Virus immunocapture provides evidence of CD8 lymphocyte-derived HIV-1 in vivo.
    AIDS. 21: 1507-13.
  2. Allen, J.S. et al. (2009) Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture.
    Diabetes. 58: 138-45.
  3. McIntosh, K. et al. (2006) The immunogenicity of human adipose-derived cells: temporal changes in vitro.
    Stem Cells. 24: 1246-53.
  4. Sengstake, S. et al. (2006) CD21 and CD62L shedding are both inducible via P2X7Rs.
    Int Immunol. 18 (7): 1171-8.
  5. Villarroel Dorrego, M. et al. (2006) Transfection of CD40 in a human oral squamous cell carcinoma keratinocyte line upregulates immune potency and costimulatory molecules.
    Br J Dermatol. 154: 231-8.
  6. Franz, B. et al. (2011) Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.
    Blood. 118: 348-57.
  7. Lacal, P.M. et al. (2013) Glucocorticoid-induced tumor necrosis factor receptor family-related ligand triggering upregulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 and promotes leukocyte adhesion.
    J Pharmacol Exp Ther. 347: 164-72.
  8. Franz, B. et al. (2011) Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.
    Blood. 118: 348-57.
  9. View The Latest Product References
  10. Girbl, T. et al. (2013) CD40-mediated activation of chronic lymphocytic leukemia cells promotes their CD44-dependent adhesion to hyaluronan and restricts CCL21-induced motility.
    Cancer Res. 73: 561-70.
  11. Hertzberg, L. et al. (2010) Down syndrome acute lymphoblastic leukemia, a highly heterogeneous disease in which aberrant expression of CRLF2 is associated with mutated JAK2: a report from the International BFM Study Group.
    Blood. 115: 1006-17.
  12. Kakko, T. et al. (2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.
    Diab Vasc Dis Res. 8: 221-8.
  13. Dorvignit, D. et al. (2012) Expression and biological characterization of an anti-CD20 biosimilar candidate antibody: a case study.
    MAbs. 4 (4): 488-96.
  14. Karlsen, M. et al. (2015) TLR-7 and -9 Stimulation of Peripheral Blood B Cells Indicate Altered TLR Signalling in Primary Sjögren's Syndrome Patients by Increased Secretion of Cytokines.
    Scand J Immunol. 82 (6): 523-31.
  15. Clark, L.E. et al. (2018) Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses.
    Nat Commun. 9 (1): 1884.
  16. Gu, Y. et al. (2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.
    Nat Commun. 10 (1): 893.
  17. Yang, C. et al. (2013) B cells promote tumor progression via STAT3 regulated-angiogenesis.
    PLoS One. 8 (5): e64159.

Flow Cytometry

RRID
AB_566591
UniProt
P15391
Entrez Gene
CD19
GO Terms
GO:0005515 protein binding
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response

MCA1940PB

0315 154335

MCA1940PBT

154336 1701

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