CD18 antibody | MEM-148

Mouse anti Human CD18 (Activation Epitope):FITC

Product Type
Monoclonal Antibody
Clone
MEM-148
Isotype
IgG1
Specificity
CD18
Region
(ACTIVATION EPITOPE)

Product Code Applications Pack Size List Price Your Price Qty
MCA2086F
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F 0.1 mg loader
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Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148 recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone MEM-148 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized by Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148 is also exposed during dissociation of the CD11/CD18 by low pH.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for CD18 antibody

  1. Drbal, K. et al. (2001) A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells.
    Blood. 98 (5): 1561-6.
  2. Luissint, A.C. et al. (2008) JAM-L-mediated leukocyte adhesion to endothelial cells is regulated in cis by alpha4beta1 integrin activation.
    J Cell Biol. 183 (6): 1159-73.
  3. Feng, C. et al. (2011) Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1.
    J Leukoc Biol. 90: 313-21.
  4. Anogianaki, A. et al. (2007) Capsaicin an irritant anti-inflammatory compound.
    J Biol Regul Homeost Agents. 21: 1-4.
  5. Arthos, J. et al. (2008) HIV-1 envelope protein binds to and signals through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
    Nat Immunol. 9: 301-9.
  6. Cairo, C.W. et al. (2006) Cytoskeletal regulation couples LFA-1 conformational changes to receptor lateral mobility and clustering.
    Immunity. 25: 297-308.
  7. Tang, X.Y. et al. (2008) Intercellular adhesion molecule-3 binding of integrin alphaL beta2 requires both extension and opening of the integrin headpiece.
    J Immunol. 180: 4793-804.
  8. Kudlová M. et al. (2007) Expression of an activated form of integrin beta2 chain CD18 in cardiac surgical operations.
    Acta Medica (Hradec Kralove). 50: 187-93.
  9. View The Latest Product References
  10. Solovjov, D.A. et al. (2005) Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2.
    J Biol Chem. 280: 1336-45.
  11. Ehirchiou, D. et al. (2005) Dual function for a unique site within the beta2I domain of integrin alphaMbeta2.
    J Biol Chem. 280: 8324-31.
  12. Cheng, M. et al. (2007) Mutation of a conserved asparagine in the I-like domain promotes constitutively active integrins alphaLbeta2 and alphaIIbbeta3.
    J Biol Chem. 282: 18225-32.
  13. Shi, M. et al. (2007) A structural hypothesis for the transition between bent and extended conformations of the leukocyte beta2 integrins.
    J Biol Chem. 282: 30198-206.
  14. Dilek, N. et al. (2013) Targeting CD28, CTLA-4 and PD-L1 costimulation differentially controls immune synapses and function of human regulatory and conventional T-cells.
    PLoS One. 8 (12): e83139.

Flow Cytometry

Immunoprecipitation

Synonyms
Integrin Beta 2 Chain
RRID
AB_323796
UniProt
P05107
Entrez Gene
ITGB2
GO Terms
GO:0001948 glycoprotein binding
GO:0007596 blood coagulation
GO:0006915 apoptosis
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0006954 inflammatory response
GO:0007159 leukocyte cell-cell adhesion
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007267 cell-cell signaling
GO:0007275 multicellular organismal development
GO:0008360 regulation of cell shape
GO:0019901 protein kinase binding
GO:0030593 neutrophil chemotaxis
GO:0050730 regulation of peptidyl-tyrosine phosphorylation
GO:0050776 regulation of immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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