CD18 antibody | Bu86

100% Secure

Mouse anti Human CD18:FITC

Product Type
Monoclonal Antibody
Clone
Bu86
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2510F F 0.1 mg
Mouse anti Human CD18 antibody, clone Bu86 recognize human CD18 (integrin beta 2 subunit), a 90 kDa type I transmembrane protein which is expressed on all leucocytes. CD18 can combine with integrin molecules CD11a – c to form heterodimers at the cell surface. These heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signalling.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05107 Related reagents
Entrez Gene
ITGB2 Related reagents
GO Terms
GO:0001948 glycoprotein binding
GO:0007596 blood coagulation
GO:0006915 apoptosis
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0006954 inflammatory response
GO:0007159 leukocyte cell-cell adhesion
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007267 cell-cell signaling
GO:0007275 multicellular organismal development
GO:0008360 regulation of cell shape
GO:0019901 protein kinase binding
GO:0030593 neutrophil chemotaxis
GO:0050730 regulation of peptidyl-tyrosine phosphorylation
GO:0050776 regulation of immune response
Regulatory
For research purposes only

Applications of CD18 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD18 antibody

  1. Garland Press, Inc. (1997) Leucocyte Typing VI. White cell differentiation antigens.
    p 355–7.