CD16b antibody | 1D3

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Mouse anti Human CD16b:FITC

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
100 Tests/2ml loader

Mouse anti human CD16b antibody, clone 1D3, recognizes the human low-affinity IgG Fc region receptor III-B. CD16b is expressed only by neutrophils, whilst CD16a is expressed by neutrophils and natural killer cells.

Product Details

Target Species
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
0.2%Bovine Serum Albumin
Human granulocytes.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guaranteed until date of expiry. Please see product label.

More Information

O75015 Related reagents
Entrez Gene
FCGR3B Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0031225 anchored to membrane
GO:0019864 IgG binding
For research purposes only

Applications of CD16b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 20ul of the suggested working dilution to label 5x105 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgM Negative Control:FITC MCA692F F 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Product Specific References

References for CD16b antibody

  1. Morris, D.L. et al. (2010) Evidence for both copy number and allelic (NA1/NA2) risk at the FCGR3B locus in systemic lupus erythematosus.
    Eur J Hum Genet. 18 (9): 1027-31.
  2. Bagaitkar, J. et al. (2010) Tobacco upregulates P. gingivalis fimbrial proteins which induce TLR2 hyposensitivity.
    PLoS One. 5(5):e9323.
  3. Hill J & Samuel JE (2011) Coxiella burnetii acid phosphatase inhibits the release of reactive oxygen intermediates in polymorphonuclear leukocytes.
    Infect Immun. 79 (1): 414-20.
  4. Larsson, N. et al. (2015) Identification of vitamin C transporters in the human airways: a cross-sectional in vivo study.
    BMJ Open. 5 (4): e006979.

Further Reading

  1. Ravetch, J.V. & Kinet, J.P. (1991) Fc receptors.
    Annu Rev Immunol. 9: 457-92.