Mouse anti human CD16b antibody, clone 1D3, recognizes the human low-affinity IgG Fc region receptor III-B. CD16b is expressed only by neutrophils, whilst CD16a is expressed by neutrophils and natural killer cells.
- Target Species
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|0.2%||Bovine Serum Albumin|
- Human granulocytes.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- GO Terms
anchored to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD16b antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 20ul of the suggested working dilution to label 5x105 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
Useful Reagents Available
Product Specific References
References for CD16b antibody
Morris, D.L. et al. (2010) Evidence for both copy number and allelic (NA1/NA2) risk at the FCGR3B locus in systemic lupus erythematosus.
Eur J Hum Genet. 18 (9): 1027-31.
Bagaitkar, J. et al. (2010) Tobacco upregulates P. gingivalis fimbrial proteins which induce TLR2 hyposensitivity.
PLoS One. 5(5):e9323.
Hill J & Samuel JE (2011) Coxiella burnetii acid phosphatase inhibits the release of reactive oxygen intermediates in polymorphonuclear leukocytes.
Infect Immun. 79 (1): 414-20.
Larsson, N. et al. (2015) Identification of vitamin C transporters in the human airways: a cross-sectional in vivo study.
BMJ Open. 5 (4): e006979.
Ravetch, J.V. & Kinet, J.P. (1991) Fc receptors.
Annu Rev Immunol. 9: 457-92.