CD167 antibody
Mouse anti CD167
Mouse anti human CD167 antibody recognizes epithelial discoidin domain-containing receptor 1, also known as CD167 antigen-like family member A, tyrosine kinase DDR or tyrosine-protein kinase CAK. This antibody has been extensively validated for western blotting using whole cell lysates.
CD167 Antibody with Control Lysate
Mouse anti human CD167 antibody recognizes epithelial discoidin domain-containing receptor 1, also known as CD167 antigen-like family member A, PTK3A protein tyrosine kinase 3A, cell adhesion kinase, DDR1, protein-tyrosine kinase RTK-6, or tyrosine kinase DDR.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG2b
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00373 | WB | 100 µl | ||||
| VMA00373KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human CD167 antibody recognizes CD167, also known as CD167 antigen-like family member A, PTK3A protein tyrosine kinase 3A, cell adhesion kinase, epithelial discoidin domain-containing receptor 1 or DDR1, mammary carcinoma kinase 10, protein-tyrosine kinase RTK-6, tyrosine kinase DDR and tyrosine-protein kinase CAK.
Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases mostly regulate cell growth, differentiation and metabolism. CD167 belongs to a subfamily of tyrosine kinase receptors activated by various types of collagen with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for CD167 (provided by RefSeq, Feb 2011).
Mouse anti Human CD167 antibody detects a band of 125 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases mostly regulate cell growth, differentiation and metabolism. CD167 belongs to a subfamily of tyrosine kinase receptors activated by various types of collagen with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for CD167 (provided by RefSeq, Feb 2011).
Mouse anti Human CD167 antibody detects a band of 125 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CD167 detects a band of approximately 125 kDa in MCF7 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant CD167
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg MCF7 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q08345 Related reagents
- Entrez Gene
- DDR1 Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of CD167 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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