CD162 antibody | TC2

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Mouse anti Human CD162:RPE

Product Type
Monoclonal Antibody
Clone
TC2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6123PE
Datasheet
F
MSDS
100 Tests/2ml loader

Mouse anti Human CD162 clone TC2, recognizes CD162 also known as P-Selectin glycoprotein ligand-1 (PSGL-1). It is a 120 kDa transmembrane protein that primarily exists as a homodimer on hematopoietic cells including myeloid, lymphoid, dendritic cells and some non-hematopoietic cells (Laszik et al. 1996).

The most well know function of CD162, when activated, is its role in inflammation in that it slows down leucocytes on the surface of activated epithelium. This occurs when one of its ligands, P-, E- or L- selectin on activated platelets or activated endothelial cells bind to its N-terminal extracellular domain. Upon P-selectin binding, a very long molecule is essentially formed which extends from the activated endothelial cells and leukocyte surface layer, These can also effectively capture circulating myeloid cells. Before a ligand can bind to CD162, the receptor must be postranslationally modified with sialylated, fucosylated O-glycans as well as sulfation at the N-terminal tyrosines (Kappelmayer & Nagy 2017). Mutations which interrupt these post translational modifications prevent ligand binding and has been shown to result in patients suffering from infections of the mucosal membrane and skin (Maly et al. 1996).

The TC2 clone has been used in flow cytometry experiments to examine the effects on CD162 expression levels on the surface of neutrophils upon treatment with histidine-rich glycoprotein which can regulate immunothrombosis and inflammation (Wake et al. 2016).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Human thymocytes
Purity
>95% by SDS PAGE

Storage Information

Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch

More Information

UniProt
Q14242
Entrez Gene
SELPLG
GO Terms
GO:0007596 blood coagulation
GO:0005102 receptor binding
GO:0005887 integral to plasma membrane
GO:0005624 membrane fraction
GO:0051635 bacterial cell surface binding
GO:0071354 cellular response to interleukin-6
Regulatory
For research purposes only

Applications of CD162 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 20ul of the undiluted reagent to label 1x106 cells in 100ul

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader