CD162 antibody | TC2
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F ResetMouse anti Human CD162:RPE
- Product Type
- Monoclonal Antibody
- Clone
- TC2
- Isotype
- IgG1
- Specificity
- CD162
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| Mouse anti Human CD162 clone TC2, recognizes CD162 also known as P-Selectin glycoprotein ligand-1 (PSGL-1). It is a 120 kDa transmembrane protein that primarily exists as a homodimer on hematopoietic cells including myeloid, lymphoid, dendritic cells and some non-hematopoietic cells (Laszik et al. 1996). The most well know function of CD162, when activated, is its role in inflammation in that it slows down leucocytes on the surface of activated epithelium. This occurs when one of its ligands, P-, E- or L- selectin on activated platelets or activated endothelial cells bind to its N-terminal extracellular domain. Upon P-selectin binding, a very long molecule is essentially formed which extends from the activated endothelial cells and leukocyte surface layer, These can also effectively capture circulating myeloid cells. Before a ligand can bind to CD162, the receptor must be postranslationally modified with sialylated, fucosylated O-glycans as well as sulfation at the N-terminal tyrosines (Kappelmayer & Nagy 2017). Mutations which interrupt these post translational modifications prevent ligand binding and has been shown to result in patients suffering from infections of the mucosal membrane and skin (Maly et al. 1996). The TC2 clone has been used in flow cytometry experiments to examine the effects on CD162 expression levels on the surface of neutrophils upon treatment with histidine-rich glycoprotein which can regulate immunothrombosis and inflammation (Wake et al. 2016). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- Human thymocytes
- Purity
- >95% by SDS PAGE
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 20ul of the undiluted reagent to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:RPE | ||||||
- Synonyms
- PSGL-1
- UniProt
- Q14242
- Entrez Gene
- SELPLG
- GO Terms
- GO:0007596 blood coagulation
- GO:0005102 receptor binding
- GO:0005887 integral to plasma membrane
- GO:0005624 membrane fraction
- GO:0051635 bacterial cell surface binding
- GO:0071354 cellular response to interleukin-6
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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