CD16 antibody | LNK16

Mouse anti Human CD16:Amethyst Orange

Product Type
Monoclonal Antibody
Clone
LNK16
Isotype
IgG1
Specificity
CD16

Product Code Applications Pack Size List Price Your Price Qty
MCA1193AMO
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F 0.1 mg loader
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Mouse anti Human CD16 antibody, clone LNK16 recognizes CD16, a 50-65 kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes.

CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996).

Target Species
Human
Product Form
Purified IgG conjugated to Amethyst Orange - liquid
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Normal human peripheral blood granulocytes.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Amethyst Orange 405 540
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

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References for CD16 antibody

  1. Tamm, A. & Schmidt, R.E. (1996) The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.
    J Immunol. 157 (4): 1576-81.
  2. Fleit, H.B. et al. (1982) Human neutrophil Fc gamma receptor distribution and structure.
    Proc Natl Acad Sci U S A. 79 (10): 3275-9.
  3. van de Winkel, J.G. & Capel, P.J. (1993) Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.
    Immunol Today. 14 (5): 215-21.
  4. Pillay, J. et al. (2012) A subset of neutrophils in human systemic inflammation inhibits T cell responses through Mac-1.
    J Clin Invest. 122: 327-36.
  5. Morris, D.L. et al. (2010) Evidence for both copy number and allelic (NA1/NA2) risk at the FCGR3B locus in systemic lupus erythematosus.
    Eur J Hum Genet. 18 (9): 1027-31.
  6. Kamp, V.M. et al. (2012) Human suppressive neutrophils CD16bright/CD62Ldim exhibit decreased adhesion.
    J Leukoc Biol. 92 (5): 1011-20.
  7. Hanna, J. et al. (2003) CXCL12 expression by invasive trophoblasts induces the specific migration of CD16- human natural killer cells.
    Blood. 102:1569-77.
  8. Moalli, F. et al. (2010) Role of complement and Fc{gamma} receptors in the protective activity of the long pentraxin PTX3 against Aspergillus fumigatus.
    Blood. 116: 5170-80.
  9. View The Latest Product References
  10. Armour, K.L. et al. (2010) Expression of human FcgammaRIIIa as a GPI-linked molecule on CHO cells to enable measurement of human IgG binding.
    J Immunol Methods. 354: 20-33.
  11. Welters, I.D. et al. (2010) Ketamine inhibits transcription factors activator protein 1 and nuclear factor-kappaB, interleukin-8 production, as well as CD11b and CD16 expression: studies in human leukocytes and leukocytic cell lines.
    Anesth Analg. 110: 934-41.
  12. Bowles, J.A. et al. (2006) Anti-CD20 monoclonal antibody with enhanced affinity for CD16 activates NK cells at lower concentrations and more effectively than rituximab.
    Blood. 108: 2648-54.
  13. Meknache, N. et al. (2009) Human basophils express the glycosylphosphatidylinositol-anchored low-affinity IgG receptor FcgammaRIIIB (CD16B).
    J Immunol. 182: 2542-50.
  14. Lee, S.F. et al. (1999) Cytokine receptor common beta chain as a potential activator of cytokine withdrawal-induced apoptosis.
    Mol Cell Biol. 19: 7399-409.
  15. Sironi, M. et al. (2006) Differential regulation of chemokine production by Fcgamma receptor engagement in human monocytes: association of CCL1 with a distinct form of M2 monocyte activation (M2b, Type 2).
    J Leukoc Biol. 80: 342-9.
  16. Weissmüller, S. et al. (2012) ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
    Blood. 119: 6268-77.
  17. Kahn, F. et al. (2008) Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response.
    PLoS Pathog. 4: e1000149.
  18. Onodera, R. et al. (2017) Anti-human neutrophil antigen-1a, -1b, and -2 antibodies in neonates and children with immune neutropenias analyzed by extracted granulocyte antigen immunofluorescence assay.
    Transfusion. 57 (11): 2586-94.

Further Reading

  1. Ravetch, J.V. & Kinet, J.P. (1991) Fc receptors.
    Annu Rev Immunol. 9: 457-92.

Flow Cytometry

Immunofluorescence

Synonyms
FcRIII
UniProt
P08637
O75015
Entrez Gene
FCGR3B
FCGR3A
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0031225 anchored to membrane
GO:0019864 IgG binding
GO:0016021 integral to membrane
GO:0050776 regulation of immune response
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