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CD16 antibody | LNK16

Mouse anti Human CD16:Alexa Fluor® 647

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 Tests/0.25ml loader
List Price Your Price
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price

Mouse anti Human CD16 antibody, clone LNK16 recognizes CD16, a 50-65 kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes.

CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996).

Target Species
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Purified IgG prepared by ion exchange chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Normal human peripheral blood granulocytes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
For research purposes only
12 months from date of despatch
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
List Price Your Price
Description Mouse IgG1 Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD16 antibody

  1. Tamm, A. & Schmidt, R.E. (1996) The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.
    J Immunol. 157 (4): 1576-81.
  2. Lee, S.F. et al. (1999) Cytokine receptor common beta chain as a potential activator of cytokine withdrawal-induced apoptosis.
    Mol Cell Biol. 19: 7399-409.
  3. Hanna, J. et al. (2003) CXCL12 expression by invasive trophoblasts induces the specific migration of CD16- human natural killer cells.
    Blood. 102:1569-77.
  4. Sironi, M. et al. (2006) Differential regulation of chemokine production by Fcgamma receptor engagement in human monocytes: association of CCL1 with a distinct form of M2 monocyte activation (M2b, Type 2).
    J Leukoc Biol. 80: 342-9.
  5. Bowles, J.A. et al. (2006) Anti-CD20 monoclonal antibody with enhanced affinity for CD16 activates NK cells at lower concentrations and more effectively than rituximab.
    Blood. 108: 2648-54.
  6. Kahn, F. et al. (2008) Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response.
    PLoS Pathog. 4: e1000149.
  7. Meknache, N. et al. (2009) Human basophils express the glycosylphosphatidylinositol-anchored low-affinity IgG receptor FcgammaRIIIB (CD16B).
    J Immunol. 182: 2542-50.
  8. Welters, I.D. et al. (2010) Ketamine inhibits transcription factors activator protein 1 and nuclear factor-kappaB, interleukin-8 production, as well as CD11b and CD16 expression: studies in human leukocytes and leukocytic cell lines.
    Anesth Analg. 110: 934-41.
  9. View The Latest Product References
  10. Armour, K.L. et al. (2010) Expression of human FcgammaRIIIa as a GPI-linked molecule on CHO cells to enable measurement of human IgG binding.
    J Immunol Methods. 354: 20-33.
  11. Moalli, F. et al. (2010) Role of complement and Fc{gamma} receptors in the protective activity of the long pentraxin PTX3 against Aspergillus fumigatus.
    Blood. 116: 5170-80.
  12. Morris, D.L. et al. (2010) Evidence for both copy number and allelic (NA1/NA2) risk at the FCGR3B locus in systemic lupus erythematosus.
    Eur J Hum Genet. 18 (9): 1027-31.
  13. Kamp, V.M. et al. (2012) Human suppressive neutrophils CD16bright/CD62Ldim exhibit decreased adhesion.
    J Leukoc Biol. 92 (5): 1011-20.
  14. Weissmüller, S. et al. (2012) ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
    Blood. 119: 6268-77.
  15. Pillay, J. et al. (2012) A subset of neutrophils in human systemic inflammation inhibits T cell responses through Mac-1.
    J Clin Invest. 122: 327-36.
  16. Onodera, R. et al. (2017) Anti-human neutrophil antigen-1a, -1b, and -2 antibodies in neonates and children with immune neutropenias analyzed by extracted granulocyte antigen immunofluorescence assay.
    Transfusion. 57 (11): 2586-94.

Further Reading

  1. Ravetch, J.V. & Kinet, J.P. (1991) Fc receptors.
    Annu Rev Immunol. 9: 457-92.
  2. van de Winkel, J.G. & Capel, P.J. (1993) Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.
    Immunol Today. 14 (5): 215-21.

Flow Cytometry


Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0031225 anchored to membrane
GO:0019864 IgG binding
GO:0016021 integral to membrane
GO:0050776 regulation of immune response


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