CD15 antibody | MEM-158
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|Mouse anti Human CD15 antibody, clone MEM-158 recognizes the human CD15 cell surface antigen, also known as Lewis X , stage-specfic embryonic antigen-1 or SSEA-1.
CD15 is a carbohydrate antigen, predominately expressed by peripheral blood granulocytes (Brackman et al.1995) but also on a variety of other normal cells and many tumors (Ohana-Malka et al. 2003). Expression of CD15 is widely used as a diagnostic indicator of Hodgkin's disease where acquisition of a sialyl group by CD15 is indicative of progression to a more disemminated form of the disease and poor prognosis (Benharroch et al. 2000).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Pig
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- MCA2458F: Purified IgM prepared by ion exchange chromatography.
- MCA2458FT: Purified IgM prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- Human granulocytes.
- Approx. Protein Concentrations
- Ig concentration 0.1 mg/ml
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
References for CD15 antibody
Brackman, D. et al. (1995) Expression of leukocyte differentiation antigens during the differentiation of HL-60 cells induced by 1,25-dihydroxyvitamin D3: comparison with the maturation of normal monocytic and granulocytic bone marrow cells.
J Leukoc Biol. 58: 547-55.
Lowdell, M. (2014) Preserved compositions of activated NK cells and methods of using the same.
US Patent US8735148 B2
Woolley, J.R. et al. (2014) Temporal leukocyte numbers and granulocyte activation in pulsatile and rotary ventricular assist device patients.
Artif Organs. 38 (6): 447-55.
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