CD15 antibody | MEM-158
Mouse anti Human CD15 antibody, clone MEM-158 recognizes the human CD15 cell surface antigen, also known as Lewis X , stage-specfic embryonic antigen-1 or SSEA-1.
CD15 is a carbohydrate antigen, predominately expressed by peripheral blood granulocytes (Brackman et al.1995) but also on a variety of other normal cells and many tumors (Ohana-Malka et al. 2003). Expression of CD15 is widely used as a diagnostic indicator of Hodgkin's disease where acquisition of a sialyl group by CD15 is indicative of progression to a more disemminated form of the disease and poor prognosis (Benharroch et al. 2000).
CD15 is a carbohydrate antigen, predominately expressed by peripheral blood granulocytes (Brackman et al.1995) but also on a variety of other normal cells and many tumors (Ohana-Malka et al. 2003). Expression of CD15 is widely used as a diagnostic indicator of Hodgkin's disease where acquisition of a sialyl group by CD15 is indicative of progression to a more disemminated form of the disease and poor prognosis (Benharroch et al. 2000).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgM prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Human granulocytes.
- Approx. Protein Concentrations
- Ig concentration 0.1 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD15 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
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Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgM Negative Control:FITC | MCA692F | F | 0.1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD15 antibody
-
Brackman, D. et al. (1995) Expression of leukocyte differentiation antigens during the differentiation of HL-60 cells induced by 1,25-dihydroxyvitamin D3: comparison with the maturation of normal monocytic and granulocytic bone marrow cells.
J Leukoc Biol. 58: 547-55. -
Lowdell, M. (2014) Preserved compositions of activated NK cells and methods of using the same.
US Patent US8735148 B2 -
Woolley, J.R. et al. (2014) Temporal leukocyte numbers and granulocyte activation in pulsatile and rotary ventricular assist device patients.
Artif Organs. 38 (6): 447-55.
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