CD15 antibody | MEM-158
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F ResetMouse anti Human CD15:FITC
- Product Type
- Monoclonal Antibody
- Clone
- MEM-158
- Isotype
- IgM
- Specificity
- CD15
Mouse anti Human CD15 antibody, clone MEM-158 recognizes the human CD15 cell surface antigen, also known as Lewis X , stage-specfic embryonic antigen-1 or SSEA-1. CD15 is a carbohydrate antigen, predominately expressed by peripheral blood granulocytes (Brackman et al.1995) but also on a variety of other normal cells and many tumors (Ohana-Malka et al. 2003). Expression of CD15 is widely used as a diagnostic indicator of Hodgkin's disease where acquisition of a sialyl group by CD15 is indicative of progression to a more disemminated form of the disease and poor prognosis (Benharroch et al. 2000). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- MCA2458F: Purified IgM prepared by ion exchange chromatography.
- MCA2458FT: Purified IgM prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Human granulocytes.
- Approx. Protein Concentrations
- Ig concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
Neat | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgM Negative Control:FITC | MCA692F | F | 0.1 mg |
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Description | Mouse IgM Negative Control:FITC |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
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Description | Human Seroblock |
References for CD15 antibody
-
Brackman, D. et al. (1995) Expression of leukocyte differentiation antigens during the differentiation of HL-60 cells induced by 1,25-dihydroxyvitamin D3: comparison with the maturation of normal monocytic and granulocytic bone marrow cells.
J Leukoc Biol. 58: 547-55. -
Lowdell, M. (2014) Preserved compositions of activated NK cells and methods of using the same.
US Patent US8735148 B2 -
Woolley, J.R. et al. (2014) Temporal leukocyte numbers and granulocyte activation in pulsatile and rotary ventricular assist device patients.
Artif Organs. 38 (6): 447-55.
- Synonyms
- Lewis X
- RRID
- AB_566529
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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