Mouse anti Human CD15 antibody, clone MEM-158
recognizes the human CD15 cell surface antigen, also known as Lewis X , stage-specfic embryonic antigen-1 or SSEA-1.
CD15 is a carbohydrate antigen, predominately expressed by peripheral blood granulocytes (Brackman et al.1995
) but also on a variety of other normal cells and many tumors (Ohana-Malka et al. 2003
). Expression of CD15 is widely used as a diagnostic indicator of Hodgkin's disease where acquisition of a sialyl group by CD15 is indicative of progression to a more disemminated form of the disease and poor prognosis (Benharroch et al. 2000
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgM prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Human granulocytes.
- Approx. Protein Concentrations
- Ig concentration 0.1 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD15 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
Useful Reagents Available
Product Specific References
References for CD15 antibody
Brackman, D. et al. (1995) Expression of leukocyte differentiation antigens during the differentiation of HL-60 cells induced by 1,25-dihydroxyvitamin D3: comparison with the maturation of normal monocytic and granulocytic bone marrow cells.
J Leukoc Biol. 58: 547-55.
Lowdell, M. (2014) Preserved compositions of activated NK cells and methods of using the same.
US Patent US8735148 B2
Woolley, J.R. et al. (2014) Temporal leukocyte numbers and granulocyte activation in pulsatile and rotary ventricular assist device patients.
Artif Organs. 38 (6): 447-55.
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra