CD15 antibody | MEM-158

Mouse anti Human CD15:FITC

Product Type
Monoclonal Antibody
Clone
MEM-158
Isotype
IgM
Specificity
CD15

Product Code Applications Pack Size List Price Your Price Qty
MCA2458F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
MCA2458FT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD15 antibody, clone MEM-158 recognizes the human CD15 cell surface antigen, also known as Lewis X , stage-specfic embryonic antigen-1 or SSEA-1.

CD15 is a carbohydrate antigen, predominately expressed by peripheral blood granulocytes (Brackman et al.1995) but also on a variety of other normal cells and many tumors (Ohana-Malka et al. 2003). Expression of CD15 is widely used as a diagnostic indicator of Hodgkin's disease where acquisition of a sialyl group by CD15 is indicative of progression to a more disemminated form of the disease and poor prognosis (Benharroch et al. 2000).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
MCA2458F: Purified IgM prepared by ion exchange chromatography.
MCA2458FT: Purified IgM prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human granulocytes.
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgM Negative Control:FITC MCA692F F 0.1 mg loader
List Price Your Price
loader
Description Mouse IgM Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD15 antibody

  1. Brackman, D. et al. (1995) Expression of leukocyte differentiation antigens during the differentiation of HL-60 cells induced by 1,25-dihydroxyvitamin D3: comparison with the maturation of normal monocytic and granulocytic bone marrow cells.
    J Leukoc Biol. 58: 547-55.
  2. Lowdell, M. (2014) Preserved compositions of activated NK cells and methods of using the same.
    US Patent US8735148 B2
  3. Woolley, J.R. et al. (2014) Temporal leukocyte numbers and granulocyte activation in pulsatile and rotary ventricular assist device patients.
    Artif Organs. 38 (6): 447-55.

Synonyms
Lewis X
RRID
AB_566529
View more products with CD15 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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