CD15 antibody | Bu28

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Mouse anti Human CD15:FITC

Mouse anti Human CD15

Product Type
Monoclonal Antibody
Clone
Bu28
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2656F F 0.1 mg
MCA2656 F 0.2 mg
Mouse anti Human CD15 antibody, clone Bu28 recognises human CD15, otherwise known as Lewis X and stage-specfic embryonic antigen-1 (SSEA-1). The CD15 antigen is itself a terminal trisaccharide, Gal-beta1-4 (Fuc-alpha1-3) GlcNAc (3-FAL), which is found on a variety of glycoproteins and glycolipids. CD15 is predominately expressed by peripheral blood granulocytes and various epithelial tissues. It plays an important role in cell adhesion and has been used as a marker for diagnosis of Hodgkin’s lymphoma.

Product Details

Target Species
Human
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgM - liquid
Preparation
Ig fraction prepared by ammonium sulphate precipitation
Preparation
Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Approx. Protein Concentrations
Ig concentration 0.1mg/ml
Approx. Protein Concentrations
Ig concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD15 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Flow Cytometry 1/100 1/200
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgM Negative Control:FITC MCA692F 0.1 mg F
Mouse IgM Negative Control MCA692 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD15 antibody

  1. Oxford University Press. (1998) .
    Leucocyte Typing IV. p798-810.