CD15 antibody | Bu28

Mouse anti Human CD15

Product Type
Monoclonal Antibody
Clone
Bu28
Isotype
IgM
Specificity
CD15

Product Code Applications Pack Size List Price Your Price Qty
MCA2656
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD15 antibody, clone Bu28 recognises human CD15, otherwise known as Lewis X and stage-specfic embryonic antigen-1 (SSEA-1). The CD15 antigen is itself a terminal trisaccharide, Gal-beta1-4 (Fuc-alpha1-3) GlcNAc (3-FAL), which is found on a variety of glycoproteins and glycolipids. CD15 is predominately expressed by peripheral blood granulocytes and various epithelial tissues. It plays an important role in cell adhesion and has been used as a marker for diagnosis of Hodgkin’s lymphoma.

Target Species
Human
Product Form
Purified IgM - liquid
Preparation
Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Approx. Protein Concentrations
Ig concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A C E P WB 1 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)
Human anti Mouse IgM:FITC HCA040F F 0.1 mg loader
List Price Your Price
loader
Description Human anti Mouse IgM:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgM Negative Control MCA692 F 100 Tests loader
List Price Your Price
loader
Description Mouse IgM Negative Control

References for CD15 antibody

  1. Oxford University Press. (1998) .
    Leucocyte Typing IV. p798-810.

Synonyms
Lewis X
RRID
AB_1055605
View more products with CD15 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products