CD15 antibody | Bu28
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Mouse anti Human CD15
- Product Type
- Monoclonal Antibody
- Clone
- Bu28
- Isotype
- IgM
- Specificity
- CD15
Antibody Quality and 
Unrivaled 
| Mouse anti Human CD15 antibody, clone Bu28 recognises human CD15, otherwise known as Lewis X and stage-specfic embryonic antigen-1 (SSEA-1). The CD15 antigen is itself a terminal trisaccharide, Gal-beta1-4 (Fuc-alpha1-3) GlcNAc (3-FAL), which is found on a variety of glycoproteins and glycolipids. CD15 is predominately expressed by peripheral blood granulocytes and various epithelial tissues. It plays an important role in cell adhesion and has been used as a marker for diagnosis of Hodgkin’s lymphoma. |
- Target Species
- Human
- Product Form
- Purified IgM - liquid
- Preparation
- Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Approx. Protein Concentrations
- Ig concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
| Immunohistology - Paraffin |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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| Description | Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | ||||||
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| Description | Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | ||||||
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| Description | Human anti Mouse IgM:FITC | ||||||
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| Mouse IgM Negative Control | MCA692 | F | 100 Tests |
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| Description | Mouse IgM Negative Control | ||||||
References for CD15 antibody
-
Oxford University Press. (1998) .
Leucocyte Typing IV. p798-810.
- Synonyms
- Lewis X
- RRID
- AB_1055605
View more products with CD15 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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