Find out more about A.I.den - our SearchBot

CD15 antibody | Bu28

100% Secure
No associated image currently available
No associated image currently available

Mouse anti Human CD15

Product Type
Monoclonal Antibody
Clone
Bu28
Isotype
IgM
Product Code Applications Pack Size List Price Quantity
MCA2656
Datasheet
F
SDS
0.2 mg loader

Mouse anti Human CD15 antibody, clone Bu28 recognises human CD15, otherwise known as Lewis X and stage-specfic embryonic antigen-1 (SSEA-1). The CD15 antigen is itself a terminal trisaccharide, Gal-beta1-4 (Fuc-alpha1-3) GlcNAc (3-FAL), which is found on a variety of glycoproteins and glycolipids. CD15 is predominately expressed by peripheral blood granulocytes and various epithelial tissues. It plays an important role in cell adhesion and has been used as a marker for diagnosis of Hodgkin’s lymphoma.

Product Details

Target Species
Human
Product Form
Purified IgM - liquid
Preparation
Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Approx. Protein Concentrations
Ig concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD15 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgM:FITC HCA040F F 0.1 mg loader
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A C E P WB 1 ml loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgM Negative Control MCA692 F 100 Tests loader

Product Specific References

References for CD15 antibody

  1. Oxford University Press. (1998) .
    Leucocyte Typing IV. p798-810.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra
View more products with CD15 specificity