CD148 antibody | MEM-CD148/05

Mouse anti Human CD148

Product Type
Monoclonal Antibody
Clone
MEM-CD148/05
Isotype
IgG2b
Specificity
CD148

Product Code Applications Pack Size List Price Your Price Qty
MCA6120
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD148 antibody, clone MEM-CD148/05 recognizes CD148, a member of the receptor protein tyrosine phosphatase family. CD148 contains an ectodomain composed of multiple fibronectin type III-like repeats, a transmembrane domain and a single catalytic phosphatase domain. CD148 is broadly expressed including in platelets, T cells, B cells, macrophages as well as in some non-hematopoietic cells. CD148 functions as a key regulator of platelet reactivity via its regulation of Src family kinases (SFKs) (Senis et al. 2009). Deletion of CD148 in mice has been shown to significantly impact platelet reactivity and thrombosis via SFK activity regulation (Ellison et al. 2010). Clone MEM-CD148/05 has been used in immunofluorescence experiments to examine the co-localization of CD148 with CD45 at the immunological synapses formed between Tim-3hiCD8+ T cells (Clayton et al. 2014).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human recombinant CD148 (amino acids 1-444)
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for CD148 antibody

  1. Senis, Y.A. et al. (2009) The tyrosine phosphatase CD148 is an essential positive regulator of platelet activation and thrombosis.
    Blood. 113 (20): 4942-54.
  2. Ellison, S. et al. (2010) CD148 enhances platelet responsiveness to collagen by maintaining a pool of active Src family kinases.
    J Thromb Haemost. 8 (7): 1575-83.
  3. Clayton, K.L. et al. (2014) T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
    J Immunol. 192 (2): 782-91.

UniProt
Q12913
Entrez Gene
PTPRJ
GO Terms
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0005911 cell-cell junction
GO:0006470 protein dephosphorylation
GO:0007267 cell-cell signaling
GO:0008013 beta-catenin binding
GO:0045295 gamma-catenin binding
GO:0070097 delta-catenin binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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