CD147 antibody

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Mouse anti CD147

CD147 antibody recognizes Basigin, also known as 5F7, collagenase stimulatory factor, extracellular matrix metalloproteinase inducer, EMMPRIN, leukocyte activation antigen M6, or CD147. The antibody has been extensively validated for western blotting using whole cell lysates.

CD147 Antibody with Control Lysate

CD147 antibody recognizes Basigin, also known as 5F7, collagenase stimulatory factor, extracellular matrix metalloproteinase inducer, EMMPRIN, leukocyte activation antigen M6, OK blood group antigen, tumor cell-derived collagenase stimulatory factor, TCSF or CD147.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00021 WB 100 µl
VMA00021KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human CD147 antibody recognizes the human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulatory factor. CD147 is a 385 amino acid single pass type 1 trans-membrane glycoprotein bearing 3 potential N-glycosylation sites, a single N-terminal (distal) Ig-like C2-type domain and a more proximal Ig-like V-type domain in its extracellular region. Mouse anti Human CD147 antibody was raised and screened against recombinant extracellular region of huCD147 expressed in CHO cells. Epitope mapping indicates that this antibody binds to an epitope within the distal Ig-like C-2 type domain of human CD147.

Mouse anti Human CD147 antibody binds to both un-stimulated and phytohemagglutinin (PHA) stimulated (activated) T lymphocytes with levels of CD147 enhanced following PHA stimulation. CD147 is also expressed by peripheral blood and endothelial cells as well as many cultured cells of hematopoietic and non-hematopoietic origin.

Mouse anti Human CD147 antibody recognizes human CD147 as a band of ~53 kDa by western blot analysis of HepG2 cell lysates under reducing conditions.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Soluble recombinant form of CD147 (CD147Rg) consisting of the cDNA coding for the hinge region, CH2 and CH3 domain of human IgG1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P35613 Related reagents
Entrez Gene
BSG Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0005537 mannose binding
GO:0006090 pyruvate metabolic process
GO:0007166 cell surface receptor linked signaling pathway
GO:0015129 lactate transmembrane transporter activity
GO:0042470 melanosome
GO:0050900 leukocyte migration
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD147 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti CD147 antibody detects a band of approximately 53 kDa in HepG2 cell lysates.
Western Blotting
Anti CD147 antibody detects a band of approximately 53 kDa in HepG2 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB