CD147 antibody

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Mouse anti CD147

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00021
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human CD147 antibody recognizes the human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulatory factor. CD147 is a 385 amino acid single pass type 1 trans-membrane glycoprotein bearing 3 potential N-glycosylation sites, a single N-terminal (distal) Ig-like C2-type domain and a more proximal Ig-like V-type domain in its extracellular region. Mouse anti Human CD147 antibody was raised and screened against recombinant extracellular region of huCD147 expressed in CHO cells. Epitope mapping indicates that this antibody binds to an epitope within the distal Ig-like C-2 type domain of human CD147.

Mouse anti Human CD147 antibody binds to both un-stimulated and phytohemagglutinin (PHA) stimulated (activated) T lymphocytes with levels of CD147 enhanced following PHA stimulation. CD147 is also expressed by peripheral blood and endothelial cells as well as many cultured cells of hematopoietic and non-hematopoietic origin.

Mouse anti Human CD147 antibody recognizes human CD147 as a band of ~53 kDa by western blot analysis of HepG2 cell lysates under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti CD147 antibody detects a band of approximately 53 kDa in HepG2 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Soluble recombinant form of CD147 (CD147Rg) consisting of the cDNA coding for the hinge region, CH2 and CH3 domain of human IgG1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P35613 Related reagents
Entrez Gene
BSG Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0005537 mannose binding
GO:0006090 pyruvate metabolic process
GO:0007166 cell surface receptor linked signaling pathway
GO:0015129 lactate transmembrane transporter activity
GO:0042470 melanosome
GO:0050900 leukocyte migration
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD147 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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