CD146 antibody | OTI2G10

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Mouse anti CD146

CD146 antibody recognizes cell surface glycoprotein MUC18, also known as Gicerin, S-endo 1 endothelial-associated antigen, cell surface glycoprotein P1H12, melanoma adhesion molecule or melanoma-associated antigen A32.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI2G10
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00201
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human CD146 antibody recognizes CD146, also known as gicerin, S-endo 1 endothelial-associated antigen, cell surface glycoprotein MUC18, cell surface glycoprotein P1H12, melanoma adhesion molecule, melanoma-associated antigen A32 and melanoma-associated antigen MUC18.

Mouse anti Human CD146 antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CD146 detects a band of approximately 120 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human CD146 (NP_006491) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P43121 Related reagents
Entrez Gene
MCAM Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0009653 anatomical structure morphogenesis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD146 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml
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