CD143 antibody | 9B9

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Mouse anti Human CD143:FITC

Mouse anti Human CD143

Product Type
Monoclonal Antibody
Clone
9B9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2057F F 0.1 mg
MCA2057 C* E F IP 1 ml
Mouse anti Human CD143 antibody, clone 9B9 recognizes human somatic CD143, also know as angiotensin - converting enzyme (ACE). CD143 exists in two forms, a ~170 kDa somatic form and a ~90 kDa germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

This antibody recognizes active ACE binding to an N-terminal domain epitope.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Hamster
Monkey
Rat
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Tissue Culture Supernatant - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human lung CD143 (Angiotensin converting enzyme).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse X63 - Ag8 - 653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P12821 Related reagents
Entrez Gene
ACE Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001822 kidney development
GO:0001974 blood vessel remodeling
GO:0002005 angiotensin catabolic process in blood
GO:0002019 regulation of renal output by angiotensin
GO:0003779 actin binding
GO:0004180 carboxypeptidase activity
GO:0008237 metallopeptidase activity
GO:0008241 peptidyl-dipeptidase activity
GO:0016021 integral to membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005768 endosome
GO:0008144 drug binding
GO:0008270 zinc ion binding
GO:0009897 external side of plasma membrane
GO:0014910 regulation of smooth muscle cell migration
GO:0019229 regulation of vasoconstriction
GO:0031404 chloride ion binding
GO:0031711 bradykinin receptor binding
GO:0032943 mononuclear cell proliferation
GO:0042312 regulation of vasodilation
GO:0042447 hormone catabolic process
GO:0043171 peptide catabolic process
GO:0050482 arachidonic acid secretion
GO:0060218 hemopoietic stem cell differentiation
Regulatory
For research purposes only

Applications of CD143 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Paraffin
ELISA
Flow Cytometry Neat 1/10
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Kidney

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD143 antibody

  1. Danilov, S. et al. (1994) Structure-function analysis of angiotensin I-converting enzyme using monoclonal antibodies. Selective inhibition of the amino-terminal active site.
    J Biol Chem. 269 (43): 26806-14.
  2. Metzger, R. et al. (2000) CD143 in the development of atherosclerosis.
    Atherosclerosis. 150 (1): 21-31.
  3. Danilov, S.M. et al. (1991) Lung is the target organ for a monoclonal antibody to angiotensin-converting enzyme.
    Lab Invest. 64 (1): 118-24.
  4. Ulrich, C. et al. (2011) Monocyte Angiotensin converting enzyme expression may be associated with atherosclerosis rather than arteriosclerosis in hemodialysis patients.
    Clin J Am Soc Nephrol. 6: 505-11.
  5. Danilov, S. et al. (1989) Radioimmunoimaging of lung vessels: an approach using indium-111-labeled monoclonal antibody to angiotensin-converting enzyme.
    J Nucl Med. 30:1686-92.
  6. Atochina, E. et al. (1998) Immunotargeting of catalase to ACE or ICAM-1 protects perfused rat lungs against oxidative stress.
    Am J Physiol. 275:L806-17.
  7. Seibert, E. et al. (2016) Association between autonomic nervous dysfunction and cellular inflammation in end-stage renal disease.
    BMC Cardiovasc Disord. 16 (1): 210.
  8. Seibert, E. et al. (2017) Vitamin D3 supplementation does not modify cardiovascular risk profile of adults with inadequate vitamin D status.
    Eur J Nutr. 56 (2): 621-634.
  9. Silva, E.A. et al. (2014) Endothelial cells expressing low levels of CD143 (ACE) exhibit enhanced sprouting and potency in relieving tissue ischemia.
    Angiogenesis. 17 (3): 617-30.
  10. Ulrich, C. et al. (2010) Circulating monocyte subpopulations with high expression of angiotensin-converting enzyme predict mortality in patients with end-stage renal disease.
    Nephrol Dial Transplant. 25 (7): 2265-72.
  11. Ulrich, C. et al. (2006) Increased expression of monocytic angiotensin-converting enzyme in dialysis patients with cardiovascular disease.
    Nephrol Dial Transplant. 21 (6): 1596-602.
  12. Nowak, K. et al. (2007) Alterations of tumor and normal tissue of human lung cancer resection specimens after isolation perfusion.
    J Physiol Pharmacol. 58 Suppl 5 (Pt 2): 501-11.
  13. Böttcher, A. et al. (2006) Angiotensin-converting enzyme signalling in human preadipocytes and adipocytes
    Open Life Sciences. 1 (2) [Epub ahead of print].
  14. Fiedler, R. et al. (2012) Randomized controlled pilot study of 2 weeks' treatment with high cutoff membrane for hemodialysis patients with elevated C-reactive protein.
    Artif Organs. 36 (10): 886-93.
  15. Eliasson, R. et al. (2013) Method and kit for cancer diagnosis.
    Patent Application: US 13/641,424. Publication number: US20130040849 A1.