CD143 antibody | 2E2
Mouse anti Human CD143 antibody, clone 2E2 recognizes human CD143, also know as angiotensin - converting enzyme (ACE). CD143 exists in two forms, a 170 kDa somatic form and a 90 kDa germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. This clone recognises denatured somatic form of ACE, binding to an epitope in the C domain, and has been reported to be active in western blotting (Balyasnikova et al. 2003).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Bovine Guinea Pig Rat Cat Pig Monkey Mouse Dog Rabbit
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
0.09% Sodium Azide
- Denatured Human kidney CD143 (angiotensin converting enzyme).
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse X63 - Ag8 - 653 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P12821 Related reagents
- Entrez Gene
- ACE Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0001822 kidney development
- GO:0001974 blood vessel remodeling
- GO:0002005 angiotensin catabolic process in blood
- GO:0002019 regulation of renal output by angiotensin
- GO:0003779 actin binding
- GO:0004180 carboxypeptidase activity
- GO:0008237 metallopeptidase activity
- GO:0008241 peptidyl-dipeptidase activity
- GO:0016021 integral to membrane
- GO:0005615 extracellular space
- GO:0005624 membrane fraction
- GO:0005768 endosome
- GO:0008144 drug binding
- GO:0008270 zinc ion binding
- GO:0009897 external side of plasma membrane
- GO:0014910 regulation of smooth muscle cell migration
- GO:0019229 regulation of vasoconstriction
- GO:0031404 chloride ion binding
- GO:0031711 bradykinin receptor binding
- GO:0032943 mononuclear cell proliferation
- GO:0042312 regulation of vasodilation
- GO:0042447 hormone catabolic process
- GO:0043171 peptide catabolic process
- GO:0050482 arachidonic acid secretion
- GO:0060218 hemopoietic stem cell differentiation
- For research purposes only
Applications of CD143 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||Neat||1/20|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney (lung in rat studies)
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for CD143 antibody
Metzger, R. et al. (1999) Angiotensin-converting enzyme in non-neoplastic kidney diseases.
Kidney Int. 56 (4): 1442-54.
Balyasnikova, I.V. et al. (2003) Monoclonal antibodies to denatured ACE (CD143) broad species specificity, reactivity on paraffin sections and detection of subtle conformational changes in the C-terminal domain of ACE.
Tissue Antigens. 61 (1): 49- 62.
Manton, R.N. et al. (2016) Angiolymphoid hyperplasia with eosinophilia developing within a port wine stain.
J Cutan Pathol. 43 (1): 53-6.
Constantino, P.B. et al. (2017) Exercise attenuates dexamethasone-induced hypertension through an improvement of baroreflex activity independently of the renin-angiotensin system.
Steroids. 128: 147-54.