CD143 antibody | 2E2

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Mouse anti Human CD143

Product Type
Monoclonal Antibody
Clone
2E2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2056 E P* WB 1 ml
Mouse anti Human CD143 antibody, clone 2E2 recognizes human CD143, also know as angiotensin - converting enzyme (ACE). CD143 exists in two forms, a 170 kDa somatic form and a 90 kDa germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. This clone recognises denatured somatic form of ACE, binding to an epitope in the C domain, and has been reported to be active in western blotting (Balyasnikova et al. 2003).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Guinea Pig
Rat
Cat
Pig
Monkey
Mouse
Dog
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Denatured Human kidney CD143 (angiotensin converting enzyme).
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse X63 - Ag8 - 653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P12821 Related reagents
Entrez Gene
ACE Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001822 kidney development
GO:0001974 blood vessel remodeling
GO:0002005 angiotensin catabolic process in blood
GO:0002019 regulation of renal output by angiotensin
GO:0003779 actin binding
GO:0004180 carboxypeptidase activity
GO:0008237 metallopeptidase activity
GO:0008241 peptidyl-dipeptidase activity
GO:0016021 integral to membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005768 endosome
GO:0008144 drug binding
GO:0008270 zinc ion binding
GO:0009897 external side of plasma membrane
GO:0014910 regulation of smooth muscle cell migration
GO:0019229 regulation of vasoconstriction
GO:0031404 chloride ion binding
GO:0031711 bradykinin receptor binding
GO:0032943 mononuclear cell proliferation
GO:0042312 regulation of vasodilation
GO:0042447 hormone catabolic process
GO:0043171 peptide catabolic process
GO:0050482 arachidonic acid secretion
GO:0060218 hemopoietic stem cell differentiation
Regulatory
For research purposes only

Applications of CD143 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 Neat 1/20
Western Blotting 1/50 1/100
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Kidney (lung in rat studies)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for CD143 antibody

  1. Metzger, R. et al. (1999) Angiotensin-converting enzyme in non-neoplastic kidney diseases.
    Kidney Int. 56 (4): 1442-54.
  2. Balyasnikova, I.V. et al. (2003) Monoclonal antibodies to denatured ACE (CD143) broad species specificity, reactivity on paraffin sections and detection of subtle conformational changes in the C-terminal domain of ACE.
    Tissue Antigens. 61 (1): 49- 62.
  3. Manton, R.N. et al. (2016) Angiolymphoid hyperplasia with eosinophilia developing within a port wine stain.
    J Cutan Pathol. 43 (1): 53-6.
  4. Constantino, P.B. et al. (2017) Exercise attenuates dexamethasone-induced hypertension through an improvement of baroreflex activity independently of the renin-angiotensin system.
    Steroids. 128: 147-54.