CD142 antibody | CLB/TF-5

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Mouse anti Human CD142:FITC

Product Type: Monoclonal Antibody
Clone: CLB/TF-5
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2548FT	F			25 µg
MCA2548F	F			0.1 mg

Mouse anti Human CD142 antibody, clone CLB/TF-5 recognizes human CD142, a 45kD cell surface glycoprotein which is otherwise known as Tissue Factor (TF). CD142 expression can be induced on monocytes, macrophages and endothelial cells by various stimuli including interleukin 1, tumour necrosis factor and endotoxin. CD142 initiates the blood clotting cascade by binding coagulation factor VIIa, which activates factor IX or factor X by specific limited proteolysis. CD142 also plays an important role in inflammation, angiogenesis, and the pathophysiology of atherosclerosis and cancer.

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.09%	Sodium Azide (NaN₃)
1%	Bovine Serum Albumin

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P13726 Related reagents
Entrez Gene: F3 Related reagents
GO Terms: GO:0001938 positive regulation of endothelial cell proliferation; GO:0002020 protease binding; GO:0002541 activation of plasma proteins involved in acute inflammatory response; GO:0002543 activation of blood coagulation via clotting cascade; GO:0016021 integral to membrane; GO:0005543 phospholipid binding; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0006919 activation of caspase activity; GO:0007598 blood coagulation, extrinsic pathway; GO:0010641 positive regulation of platelet-derived growth factor receptor signaling pathway; GO:0031012 extracellular matrix; GO:0031233 intrinsic to external side of plasma membrane; GO:0043498 cell surface binding; GO:0045766 positive regulation of angiogenesis; GO:0050927 positive regulation of positive chemotaxis; GO:0051897 positive regulation of protein kinase B signaling cascade
Regulatory: For research purposes only

Applications of CD142 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD142 antibody

VanDerPutten, R.F. et al. (2005) High-affinity antibodies in a new immunoassay for plasma tissue factor: reduction in apparent intra-individual variation.
Clin Chem Lab Med. 43 (12): 1386-91.
Mobarrez, F. et al. (2010) A multicolor flow cytometric assay for measurement of platelet-derived microparticles.
Thromb Res. 125: e110-6.
Tehrani, S. et al. (2010) Atorvastatin has antithrombotic effects in patients with type 1 diabetes and dyslipidemia.
Thromb Res. 126: e225-31.
Aass, H.C. et al. (2011) Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis.
Cytometry A. 79: 990-9.
Trummer, A. et al. (2008) Isotype controls in phenotyping and quantification of microparticles: a major source of error and how to evade it.
Thromb Res. 122: 691-700.

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