Mouse anti Human CD142 antibody, clone CLB/TF-5 recognizes human CD142, a 45kD cell surface glycoprotein which is otherwise known as Tissue Factor (TF). CD142 expression can be induced on monocytes, macrophages and endothelial cells by various stimuli including interleukin 1, tumour necrosis factor and endotoxin. CD142 initiates the blood clotting cascade by binding coagulation factor VIIa, which activates factor IX or factor X by specific limited proteolysis. CD142 also plays an important role in inflammation, angiogenesis, and the pathophysiology of atherosclerosis and cancer.
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
|0.09%||Sodium Azide (NaN3)|
|1%||Bovine Serum Albumin|
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
positive regulation of endothelial cell proliferation
activation of plasma proteins involved in acute inflammatory response
activation of blood coagulation via clotting cascade
integral to membrane
activation of caspase activity
blood coagulation, extrinsic pathway
positive regulation of platelet-derived growth factor receptor signaling pathway
intrinsic to external side of plasma membrane
cell surface binding
positive regulation of angiogenesis
positive regulation of positive chemotaxis
positive regulation of protein kinase B signaling cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD142 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Negative Isotype Controls Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for CD142 antibody
VanDerPutten, R.F. et al. (2005) High-affinity antibodies in a new immunoassay for plasma tissue factor: reduction in apparent intra-individual variation.
Clin Chem Lab Med. 43 (12): 1386-91.
Mobarrez, F. et al. (2010) A multicolor flow cytometric assay for measurement of platelet-derived microparticles.
Thromb Res. 125: e110-6.
Tehrani, S. et al. (2010) Atorvastatin has antithrombotic effects in patients with type 1 diabetes and dyslipidemia.
Thromb Res. 126: e225-31.
Aass, H.C. et al. (2011) Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis.
Cytometry A. 79: 990-9.
Trummer, A. et al. (2008) Isotype controls in phenotyping and quantification of microparticles: a major source of error and how to evade it.
Thromb Res. 122: 691-700.
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