CD142 antibody | CLB/TF-5

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Mouse anti Human CD142:FITC

Product Type
Monoclonal Antibody
Clone
CLB/TF-5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2548FT F 25 µg
MCA2548F F 0.1 mg
Mouse anti Human CD142 antibody, clone CLB/TF-5 recognizes human CD142, a 45kD cell surface glycoprotein which is otherwise known as Tissue Factor (TF). CD142 expression can be induced on monocytes, macrophages and endothelial cells by various stimuli including interleukin 1, tumour necrosis factor and endotoxin. CD142 initiates the blood clotting cascade by binding coagulation factor VIIa, which activates factor IX or factor X by specific limited proteolysis. CD142 also plays an important role in inflammation, angiogenesis, and the pathophysiology of atherosclerosis and cancer.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P13726 Related reagents
Entrez Gene
F3 Related reagents
GO Terms
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002020 protease binding
GO:0002541 activation of plasma proteins involved in acute inflammatory response
GO:0002543 activation of blood coagulation via clotting cascade
GO:0016021 integral to membrane
GO:0005543 phospholipid binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006919 activation of caspase activity
GO:0007598 blood coagulation, extrinsic pathway
GO:0010641 positive regulation of platelet-derived growth factor receptor signaling pathway
GO:0031012 extracellular matrix
GO:0031233 intrinsic to external side of plasma membrane
GO:0043498 cell surface binding
GO:0045766 positive regulation of angiogenesis
GO:0050927 positive regulation of positive chemotaxis
GO:0051897 positive regulation of protein kinase B signaling cascade
Regulatory
For research purposes only

Applications of CD142 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD142 antibody

  1. VanDerPutten, R.F. et al. (2005) High-affinity antibodies in a new immunoassay for plasma tissue factor: reduction in apparent intra-individual variation.
    Clin Chem Lab Med. 43 (12): 1386-91.
  2. Mobarrez, F. et al. (2010) A multicolor flow cytometric assay for measurement of platelet-derived microparticles.
    Thromb Res. 125: e110-6.
  3. Tehrani, S. et al. (2010) Atorvastatin has antithrombotic effects in patients with type 1 diabetes and dyslipidemia.
    Thromb Res. 126: e225-31.
  4. Aass, H.C. et al. (2011) Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis.
    Cytometry A. 79: 990-9.
  5. Trummer, A. et al. (2008) Isotype controls in phenotyping and quantification of microparticles: a major source of error and how to evade it.
    Thromb Res. 122: 691-700.