Mouse anti Human CD142 antibody, clone CLB/TF-1 recognizes human CD142, a 45 kDa cell surface glycoprotein which is otherwise known as Tissue Factor (TF). CD142 expression can be induced on monocytes, macrophages and endothelial cells by various stimuli including interleukin 1, tumour necrosis factor and endotoxin. CD142 initiates the blood clotting cascade by binding coagulation factor VIIa, which activates factor IX or factor X by specific limited proteolysis. CD142 also plays an important role in inflammation, angiogenesis, and the pathophysiology of atherosclerosis and cancer.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
positive regulation of endothelial cell proliferation
activation of plasma proteins involved in acute inflammatory response
activation of blood coagulation via clotting cascade
integral to membrane
activation of caspase activity
blood coagulation, extrinsic pathway
positive regulation of platelet-derived growth factor receptor signaling pathway
intrinsic to external side of plasma membrane
cell surface binding
positive regulation of angiogenesis
positive regulation of positive chemotaxis
positive regulation of protein kinase B signaling cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD142 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD142 antibody
van der Putten, R.F. et al. (2005) High-affinity antibodies in a new immunoassay for plasma tissue factor: reduction in apparent intra-individual variation.
Clin Chem Lab Med. 43 (12): 1386-91.
van der Putten, R.F. et al. (2005) State and diagnostic value of plasma tissue factor in early-hospitalised patients with chest pain.
Br J Haematol. 131 (1): 91-9.