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CD141 antibody | QBEND/40

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Anti Human CD141 Antibody, clone QBEND/40 thumbnail image 1 Anti Human CD141 Antibody, clone QBEND/40 thumbnail image 2 Anti Human CD141 Antibody, clone QBEND/40 thumbnail image 3 Anti Human CD141 Antibody, clone QBEND/40 thumbnail image 4 Anti Human CD141 Antibody, clone QBEND/40 thumbnail image 5 Anti Human CD141 Antibody, clone QBEND/40 thumbnail image 6 Anti Human CD141 Antibody, clone QBEND/40 thumbnail image 7 Anti Human CD141 Antibody, clone QBEND/40 thumbnail image 8
Anti Human CD141 Antibody, clone QBEND/40 gallery image 1
Staining of human lymph node. Formalin fixed, paraffin processed tissue with Mouse anti Human CD141, QBEND/40 (MCA641T).
Anti Human CD141 Antibody, clone QBEND/40 gallery image 2
Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD141 antibody, clone QBEND/40 (MCA641) followed by the HISTAR detection system (STAR3000). Low power.
Anti Human CD141 Antibody, clone QBEND/40 gallery image 3
Immunoperoxidase staining of human Tonsil cryosection with Mouse anti Human CD141 antibody, clone QBEND/40 (MCA641) followed by the HISTAR detection system (STAR3000). Low power.
Anti Human CD141 Antibody, clone QBEND/40 gallery image 4
Immunoperoxidase staining of human Tonsil cryosection with Mouse anti Human CD141 antibody, clone QBEND/40 (MCA641) followed by the HISTAR detection system (STAR3000). Medium power.
Anti Human CD141 Antibody, clone QBEND/40 gallery image 5
Immunoperoxidase staining of human Tonsil cryosection with Mouse anti Human CD141 antibody, clone QBEND/40 (MCA641) followed by the HISTAR detection system (STAR3000). High power.
Anti Human CD141 Antibody, clone QBEND/40 gallery image 6
Immunoperoxidase staining of human Tonsil cryosection with Mouse anti Human CD141 antibody, clone QBEND/40 (MCA641) followed by the HISTAR detection system (STAR3000). High power.
Anti Human CD141 Antibody, clone QBEND/40 gallery image 7
Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD141 antibody, clone QBEND/40 (MCA641), red in A and Mouse anti Human CD21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power.
Anti Human CD141 Antibody, clone QBEND/40 gallery image 8
Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD141 antibody, clone QBEND/40 (MCA641), red in A and Mouse anti Human CD21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power.

Mouse anti Human CD141

Product Type
Monoclonal Antibody
Clone
QBEND/40
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA641
Datasheet
C F P*
SDS
1 ml loader

MCA641T
Datasheet
C F P*
SDS
0.1 ml loader

Mouse anti Human CD141 antibody, clone QBEND/40 recognizes human CD141, also known as thrombomodulin. Mouse anti Human CD141 antibody, clone QBEND/40 all human endothelium tested, but does not inhibit thrombomodulin dependent activation of protein C.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
Pack Size: 1 ml
0.1%Sodium Azide
Pack Size: 0.1 ml
0.01%Sodium Azide
Immunogen
Human endothelium derived thrombomodulin.

Storage Information

Storage
Pack Size: 1 ml
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.1 ml
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P07204
Entrez Gene
THBD
GO Terms
GO:0005515 protein binding
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0009986 cell surface
GO:0010544 negative regulation of platelet activation
GO:0050900 leukocyte migration
GO:0051918 negative regulation of fibrinolysis
Regulatory
For research purposes only

Applications of CD141 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen 1/10 1/100
Immunohistology - Paraffin 1 1/10 1/50
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Lymph node
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for CD141 antibody

  1. Drake, B.L. & Loke, Y.W. (1991) Isolation of endothelial cells from human first trimester decidua using immunomagnetic beads.
    Hum Reprod. 6 (8): 1156-9.
  2. Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
    Transplantation. 89:409-16.
  3. Monsinjon, T. et al. (2003) Regulation by complement C3a and C5a anaphylatoxins of cytokine production in human umbilical vein endothelial cells.
    FASEB J. 17: 1003-14.
  4. Yockman, J.W. et al. (2009) Polymeric gene delivery of ischemia-inducible VEGF significantly attenuates infarct size and apoptosis following myocardial infarct.
    Gene Ther. 16: 127-35.
  5. Holmén, C. et al. (2005) Heterogeneity of human nasal vascular and sinusoidal endothelial cells from the inferior turbinate.
    Am J Respir Cell Mol Biol. 32: 18-27.
  6. Yamada, A. et al. (2012) Acute regeneration and chronic acellular transformation of rabbit cryopreserved aortic allografts.
    Acta Histochem Cytochem. 45: 177-86.
  7. Kaija, H. et al. (2014) Changes in cardiac thrombomodulin and heat shock transcription factor 1 expression and peripheral thrombomodulin and catecholamines during hypothermia in rats.
    Stress. 17: 504-11.
  8. Chu, C.C. et al. (2012) Resident CD141 (BDCA3)+ dendritic cells in human skin produce IL-10 and induce regulatory T cells that suppress skin inflammation.
    J Exp Med. 209: 935-45.
  9. Kaija, H.M. et al. (2012) Stress-specific responses of p21 expression: implication of transcript variant p21 alt-a in long-term hypoxia.
    J Cell Biochem. 113: 544-52.
  10. Pakanen, L. et al. (2015) Victims of lethal hypothermia have decreased levels of thrombomodulin in myocardium and urine.
    Int J Legal Med. 129 (2): 289-96.
  11. Jonigk, D. et al. (2011) Plexiform lesions in pulmonary arterial hypertension composition, architecture, and microenvironment.
    Am J Pathol. 179 (1): 167-79.

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