CD141 antibody | QBEND/40
Mouse anti Human CD141 antibody, clone QBEND/40 recognizes human CD141, also known as thrombomodulin. Mouse anti Human CD141 antibody, clone QBEND/40 all human endothelium tested, but does not inhibit thrombomodulin dependent activation of protein C.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- Pack Size: 1 ml
0.1% Sodium Azide Pack Size: 0.1 ml0.01% Sodium Azide - Immunogen
- Human endothelium derived thrombomodulin.
Storage Information
- Storage
- Pack Size: 1 mlStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mlStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P07204 Related reagents
- Entrez Gene
- THBD Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0005509 calcium ion binding
- GO:0007596 blood coagulation
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0009986 cell surface
- GO:0010544 negative regulation of platelet activation
- GO:0050900 leukocyte migration
- GO:0051918 negative regulation of fibrinolysis
- Regulatory
- For research purposes only
Applications of CD141 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Immunohistology - Frozen | 1/10 Pack Size: 1 ml 1/10 Pack Size: 0.1 ml |
1/100 | |
| Immunohistology - Paraffin 1 | 1/10 Pack Size: 1 ml 1/10 Pack Size: 0.1 ml |
1/50 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Lymph node
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F |
Application Based External Images
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Product Specific References
References for CD141 antibody
-
Drake, B.L. et al. (1991) Isolation of endothelial cells from human first trimester decidua using immunomagnetic beads.
Hum. Reprod. 6: 1156-1159. -
Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
Transplantation. 89:409-16. -
Monsinjon, T. et al. (2003) Regulation by complement C3a and C5a anaphylatoxins of cytokine production in human umbilical vein endothelial cells.
FASEB J. 17: 1003-14. -
Yockman, J.W. et al. (2009) Polymeric gene delivery of ischemia-inducible VEGF significantly attenuates infarct size and apoptosis following myocardial infarct.
Gene Ther. 16: 127-35. -
Holmén, C. et al. (2005) Heterogeneity of human nasal vascular and sinusoidal endothelial cells from the inferior turbinate.
Am J Respir Cell Mol Biol. 32: 18-27. -
Yamada, A. et al. (2012) Acute regeneration and chronic acellular transformation of rabbit cryopreserved aortic allografts.
Acta Histochem Cytochem. 45: 177-86. -
Kaija, H. et al. (2014) Changes in cardiac thrombomodulin and heat shock transcription factor 1 expression and peripheral thrombomodulin and catecholamines during hypothermia in rats.
Stress. 17: 504-11. -
Chu, C.C. et al. (2012) Resident CD141 (BDCA3)+ dendritic cells in human skin produce IL-10 and induce regulatory T cells that suppress skin inflammation.
J Exp Med. 209: 935-45. -
Kaija, H.M. et al. (2012) Stress-specific responses of p21 expression: implication of transcript variant p21 alt-a in long-term hypoxia.
J Cell Biochem. 113: 544-52. -
Pakanen, L. et al. (2015) Victims of lethal hypothermia have decreased levels of thrombomodulin in myocardium and urine.
Int J Legal Med. 129 (2): 289-96. -
Jonigk, D. et al. (2011) Plexiform lesions in pulmonary arterial hypertension composition, architecture, and microenvironment.
Am J Pathol. 179 (1): 167-79.
