CD141 antibody | QBEND/40

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Mouse anti Human CD141

Product Type
Monoclonal Antibody
Clone
QBEND/40
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA641T C F P* 0.1 ml
MCA641 C F P* 1 ml
Mouse anti Human CD141 antibody, clone QBEND/40 recognizes human CD141, also known as thrombomodulin. Mouse anti Human CD141 antibody, clone QBEND/40 all human endothelium tested, but does not inhibit thrombomodulin dependent activation of protein C.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
Pack Size: 0.1 ml
0.01%Sodium Azide
Pack Size: 1 ml
0.1%Sodium Azide
Immunogen
Human endothelium derived thrombomodulin.

Storage Information

Storage
Pack Size: 0.1 ml
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 1 ml
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P07204 Related reagents
Entrez Gene
THBD Related reagents
GO Terms
GO:0005515 protein binding
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0009986 cell surface
GO:0010544 negative regulation of platelet activation
GO:0050900 leukocyte migration
GO:0051918 negative regulation of fibrinolysis
Regulatory
For research purposes only

Applications of CD141 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen 1/10 Pack Size: 0.1 ml
1/10 Pack Size: 1 ml
1/100
Immunohistology - Paraffin 1 1/10 Pack Size: 0.1 ml
1/10 Pack Size: 1 ml
1/50
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Lymph node

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for CD141 antibody

  1. Drake, B.L. et al. (1991) Isolation of endothelial cells from human first trimester decidua using immunomagnetic beads.
    Hum. Reprod. 6: 1156-1159.
  2. Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
    Transplantation. 89:409-16.
  3. Monsinjon, T. et al. (2003) Regulation by complement C3a and C5a anaphylatoxins of cytokine production in human umbilical vein endothelial cells.
    FASEB J. 17: 1003-14.
  4. Yockman, J.W. et al. (2009) Polymeric gene delivery of ischemia-inducible VEGF significantly attenuates infarct size and apoptosis following myocardial infarct.
    Gene Ther. 16: 127-35.
  5. Holmén, C. et al. (2005) Heterogeneity of human nasal vascular and sinusoidal endothelial cells from the inferior turbinate.
    Am J Respir Cell Mol Biol. 32: 18-27.
  6. Yamada, A. et al. (2012) Acute regeneration and chronic acellular transformation of rabbit cryopreserved aortic allografts.
    Acta Histochem Cytochem. 45: 177-86.
  7. Kaija, H. et al. (2014) Changes in cardiac thrombomodulin and heat shock transcription factor 1 expression and peripheral thrombomodulin and catecholamines during hypothermia in rats.
    Stress. 17: 504-11.
  8. Chu, C.C. et al. (2012) Resident CD141 (BDCA3)+ dendritic cells in human skin produce IL-10 and induce regulatory T cells that suppress skin inflammation.
    J Exp Med. 209: 935-45.
  9. Kaija, H.M. et al. (2012) Stress-specific responses of p21 expression: implication of transcript variant p21 alt-a in long-term hypoxia.
    J Cell Biochem. 113: 544-52.
  10. Pakanen, L. et al. (2015) Victims of lethal hypothermia have decreased levels of thrombomodulin in myocardium and urine.
    Int J Legal Med. 129 (2): 289-96.
  11. Jonigk, D. et al. (2011) Plexiform lesions in pulmonary arterial hypertension composition, architecture, and microenvironment.
    Am J Pathol. 179 (1): 167-79.