CD140a antibody | 16A1

Mouse anti Human CD140a

Product Type
Monoclonal Antibody
Clone
16A1
Isotype
IgG1
Specificity
CD140a

Product Code Applications Pack Size List Price Your Price Qty
MCA6118
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IC 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD140a, clone 16A1 recognizes CD140a also known as platelet-derived growth factor receptor alpha (PDGFR-α), is a 180 kDa single pass type I transmembrane glycoprotein and a member of the receptor tyrosine kinases (Westermark 1995).

CD140a is largely expressed by mesenchymal cells and plays a key role in embryonic organogenesis and development via regulation of differentiation, migration pathways. (Hoch & Sorian 2003). Upregulation of this receptor has also been associated with various diseases including atherosclerosis, rheumatoid arthritis and nephritis (Heldin & Westermark 1999).

Upon binding of one of its ligands, PDGF-AA, PDGF-BB or PDGF-AB, the receptor forms either a homo- or hetero-dimer with CD140b. This induces autophosphorylation of the receptors intracellular tyrosine residues which activates downstream signaling pathways such as Ras-MAPK, PI3K and PLC-ϒ (Andrae et al. 2008).

This clone 16A1 has been used in immunocytochemistry experiments to examine the co-localization of CD140a with tryptophan hydroxylase-2 in the myenteric plexus (Coyle et al. 2016).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
CD140a-transfected NIH 3T3 cells
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/50
Immunocytochemistry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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References for CD140a antibody

  1. Westermark, B. (1995) [Platelet-derived growth factor. A growth factor of great physiopathological importance].
    Lakartidningen. 92 (23): 2397-401.
  2. Heldin, C.H. & Westermark, B. (1999) Mechanism of action and in vivo role of platelet-derived growth factor.
    Physiol Rev. 79 (4): 1283-316.
  3. Andrae, J. et al. (2008) Role of platelet-derived growth factors in physiology and medicine.
    Genes Dev. 22 (10): 1276-312.
  4. Coyle, D. et al. (2016) Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung's-associated enterocolitis.
    World J Gastroenterol. 22 (19): 4662-72.

Synonyms
PDGFR-α
UniProt
P16234
Entrez Gene
PDGFRA
GO Terms
GO:0001775 cell activation
GO:0005018 platelet-derived growth factor alpha-receptor activity
GO:0005021 vascular endothelial growth factor receptor activity
GO:0005887 integral to plasma membrane
GO:0005161 platelet-derived growth factor receptor binding
GO:0005524 ATP binding
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0030335 positive regulation of cell migration
GO:0042803 protein homodimerization activity
GO:0045740 positive regulation of DNA replication
GO:0046777 protein autophosphorylation
GO:0048146 positive regulation of fibroblast proliferation
GO:0048407 platelet-derived growth factor binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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