CD140a antibody | 16A1
Mouse anti Human CD140a:APC
Mouse anti Human CD140a
Mouse anti Human CD140a:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 16A1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA6118APC | F | 100 Tests/1ml |
|
|
| MCA6118 | F IC | 0.1 mg |
|
|
| MCA6118PE | F | 100 Tests/2ml |
|
Mouse anti Human CD140a, clone 16A1 recognizes CD140a also known as platelet-derived growth factor receptor alpha (PDGFR-α), is a 180 kDa single pass type I transmembrane glycoprotein and a member of the receptor tyrosine kinases (Westermark 1995).
CD140a is largely expressed by mesenchymal cells and plays a key role in embryonic organogenesis and development via regulation of differentiation, migration pathways. (Hoch & Sorian 2003). Upregulation of this receptor has also been associated with various diseases including atherosclerosis, rheumatoid arthritis and nephritis (Heldin & Westermark 1999).
Upon binding of one of its ligands, PDGF-AA, PDGF-BB or PDGF-AB, the receptor forms either a homo- or hetero-dimer with CD140b. This induces autophosphorylation of the receptors intracellular tyrosine residues which activates downstream signaling pathways such as Ras-MAPK, PI3K and PLC-ϒ (Andrae et al. 2008).
This clone 16A1 has been used in immunocytochemistry experiments to examine the co-localization of CD140a with tryptophan hydroxylase-2 in the myenteric plexus (Coyle et al. 2016).
CD140a is largely expressed by mesenchymal cells and plays a key role in embryonic organogenesis and development via regulation of differentiation, migration pathways. (Hoch & Sorian 2003). Upregulation of this receptor has also been associated with various diseases including atherosclerosis, rheumatoid arthritis and nephritis (Heldin & Westermark 1999).
Upon binding of one of its ligands, PDGF-AA, PDGF-BB or PDGF-AB, the receptor forms either a homo- or hetero-dimer with CD140b. This induces autophosphorylation of the receptors intracellular tyrosine residues which activates downstream signaling pathways such as Ras-MAPK, PI3K and PLC-ϒ (Andrae et al. 2008).
This clone 16A1 has been used in immunocytochemistry experiments to examine the co-localization of CD140a with tryptophan hydroxylase-2 in the myenteric plexus (Coyle et al. 2016).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- CD140a-transfected NIH 3T3 cells
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P16234
- Entrez Gene
- PDGFRA
- GO Terms
- GO:0001775 cell activation
- GO:0005018 platelet-derived growth factor alpha-receptor activity
- GO:0005021 vascular endothelial growth factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005161 platelet-derived growth factor receptor binding
- GO:0005524 ATP binding
- GO:0018108 peptidyl-tyrosine phosphorylation
- GO:0030335 positive regulation of cell migration
- GO:0042803 protein homodimerization activity
- GO:0045740 positive regulation of DNA replication
- GO:0046777 protein autophosphorylation
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0048407 platelet-derived growth factor binding
- Regulatory
- For research purposes only
Applications of CD140a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/50 | |
| Immunocytochemistry | |||
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the undiluted reagent to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 20ul of the undiluted reagent to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:APC | MCA928APC | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Product Specific References
References for CD140a antibody
-
Westermark, B. (1995) [Platelet-derived growth factor. A growth factor of great physiopathological importance].
Lakartidningen. 92 (23): 2397-401. -
Heldin, C.H. & Westermark, B. (1999) Mechanism of action and in vivo role of platelet-derived growth factor.
Physiol Rev. 79 (4): 1283-316. -
Andrae, J. et al. (2008) Role of platelet-derived growth factors in physiology and medicine.
Genes Dev. 22 (10): 1276-312. -
Coyle, D. et al. (2016) Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung's-associated enterocolitis.
World J Gastroenterol. 22 (19): 4662-72.
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