CD140a antibody | 16A1
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F ResetMouse anti Human CD140a
- Product Type
- Monoclonal Antibody
- Clone
- 16A1
- Isotype
- IgG1
- Specificity
- CD140a
Antibody Quality and Performance Guarantee
| Mouse anti Human CD140a, clone 16A1 recognizes CD140a also known as platelet-derived growth factor receptor alpha (PDGFR-α), is a 180 kDa single pass type I transmembrane glycoprotein and a member of the receptor tyrosine kinases (Westermark 1995). CD140a is largely expressed by mesenchymal cells and plays a key role in embryonic organogenesis and development via regulation of differentiation, migration pathways. (Hoch & Sorian 2003). Upregulation of this receptor has also been associated with various diseases including atherosclerosis, rheumatoid arthritis and nephritis (Heldin & Westermark 1999). Upon binding of one of its ligands, PDGF-AA, PDGF-BB or PDGF-AB, the receptor forms either a homo- or hetero-dimer with CD140b. This induces autophosphorylation of the receptors intracellular tyrosine residues which activates downstream signaling pathways such as Ras-MAPK, PI3K and PLC-ϒ (Andrae et al. 2008). This clone 16A1 has been used in immunocytochemistry experiments to examine the co-localization of CD140a with tryptophan hydroxylase-2 in the myenteric plexus (Coyle et al. 2016). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- CD140a-transfected NIH 3T3 cells
- Purity
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/50 |
| Immunocytochemistry | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD140a antibody
-
Westermark, B. (1995) [Platelet-derived growth factor. A growth factor of great physiopathological importance].
Lakartidningen. 92 (23): 2397-401. -
Heldin, C.H. & Westermark, B. (1999) Mechanism of action and in vivo role of platelet-derived growth factor.
Physiol Rev. 79 (4): 1283-316. -
Andrae, J. et al. (2008) Role of platelet-derived growth factors in physiology and medicine.
Genes Dev. 22 (10): 1276-312. -
Coyle, D. et al. (2016) Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung's-associated enterocolitis.
World J Gastroenterol. 22 (19): 4662-72.
- Synonyms
- PDGFR-α
- UniProt
- P16234
- Entrez Gene
- PDGFRA
- GO Terms
- GO:0001775 cell activation
- GO:0005018 platelet-derived growth factor alpha-receptor activity
- GO:0005021 vascular endothelial growth factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005161 platelet-derived growth factor receptor binding
- GO:0005524 ATP binding
- GO:0018108 peptidyl-tyrosine phosphorylation
- GO:0030335 positive regulation of cell migration
- GO:0042803 protein homodimerization activity
- View More GO Terms
- GO:0045740 positive regulation of DNA replication
- GO:0046777 protein autophosphorylation
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0048407 platelet-derived growth factor binding
MCA6118
149840
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