CD137 antibody | JG1.6A
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Rat anti Human CD137
- Product Type
- Monoclonal Antibody
- Clone
- JG1.6A
- Isotype
- IgG2a
- Specificity
- CD137
Antibody Quality and Performance Guarantee
| Rat anti Human CD137 antibody, clone JG1.6A recognizes human CD137, an 83kD type I integral membrane protein, otherwise known as 4-1BB. The CD137 molecule is primarily expressed on activated T-cells. It is a costimulatory member of the tumour necrosis receptor family and regulates T-cell activation and proliferation. Rat anti Human CD137 antibody, clone JG1.6A induces γ-interferon activity in isolated T-cells as measured by ELISA (Rajendran et al. 2019). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant human CD137-Fc.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Lymph node cells from immunised Lou rat were fused with cells of the mouse NS-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Further Reading
-
Pollok, K.E. et al. (1993) Inducible T cell antigen 4-1BB. Analysis of expression and function.
J Immunol. 150 (3): 771-81. -
Alderson, M.R. et al. (1994) Molecular and biological characterization of human 4-1BB and its ligand.
Eur J Immunol. 24 (9): 2219-27. -
Schwarz, H. et al. (1997) CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies.
Biochem Biophys Res Commun. 235 (3): 699-703. -
Cannons, J.L. et al. (2001) 4-1BB ligand induces cell division, sustains survival, and enhances effector function of CD4 and CD8 T cells with similar efficacy.
J Immunol. 167 (3): 1313-24.
- Synonyms
- 4-1BB Ligand Receptor
- RRID
- AB_844478
- UniProt
- Q07011
- Entrez Gene
- TNFRSF9
- GO Terms
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006917 induction of apoptosis
- GO:0008285 negative regulation of cell proliferation
MCA2653
153308
166981
170330
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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