CD134 antibody | Ber-ACT35

Mouse anti Human CD134

Product Type
Monoclonal Antibody
Clone
Ber-ACT35
Isotype
IgG1
Specificity
CD134

Product Code Applications Pack Size List Price Your Price Qty
MCA6116
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IP P WB 0.1 mg loader
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loader
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Mouse anti Human CD134, clone Ber-ACT35 recognizes CD134 also known as OX40 and TNFRSF4, a 50 kDa glycoprotein. CD134 is a type I transmembrane protein and a member of the tumor necrosis factor superfamily. CD134 is primarily expressed by activated T cells, although the level of expression differs according to T-cell subpopulations (Buchan et al. 2018).

OX40L also known as CD252 and TNFSF4 is the ligand of CD134. Upon ligand binding, the receptor recruits TNF receptor associated factor (TRAF) proteins which triggers downstream signaling events including NFκB pathways. This activation can regulate the number of effector T cells that accumulate in primary and secondary responses and determine the frequency of T cells that are generated (Croft 2010). Mutations affecting CD134 signaling has been linked with Kaposi sarcoma as well as susceptibility to infections.

This clone Ber-ACT35 has been used in flow cytometry experiments to measure D134 expression levels on tumor-infiltrating Treg cells in mouse and human samples (Vargas et al. 2018).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
HTLV 1-transformed HUT-102 cells
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for CD134 antibody

  1. ArceVargas, F. et al. (2018) Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.
    Cancer Cell. 33 (4): 649-663.e4.
  2. Buchan, S.L. et al. (2018) The immunobiology of CD27 and OX40 and their potential as targets for cancer immunotherapy.
    Blood. 131 (1): 39-48.
  3. Croft, M. (2010) Control of immunity by the TNFR-related molecule OX40 (CD134).
    Annu Rev Immunol. 28: 57-78.

Synonyms
OX40
TNFRSF4
UniProt
P43489
Entrez Gene
TNFRSF4
GO Terms
GO:0005031 tumor necrosis factor receptor activity
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0030890 positive regulation of B cell proliferation
GO:0032582 negative regulation of gene-specific transcription
GO:0042098 T cell proliferation
GO:0043433 negative regulation of transcription factor activity
GO:0051024 positive regulation of immunoglobulin secretion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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