CD134 antibody | Ber-ACT35

Mouse anti Human CD134:APC
Mouse anti Human CD134:FITC
Mouse anti Human CD134
Mouse anti Human CD134:RPE
- Product Type
- Monoclonal Antibody
- Clone
- Ber-ACT35
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA6116APC | F | 100 Tests/1ml |
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MCA6116F | F | 100 Tests/400µl |
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MCA6116 | C E F IP P WB | 0.1 mg |
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MCA6116PE | F | 100 Tests/1ml |
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OX40L also known as CD252 and TNFSF4 is the ligand of CD134. Upon ligand binding, the receptor recruits TNF receptor associated factor (TRAF) proteins which triggers downstream signaling events including NFκB pathways. This activation can regulate the number of effector T cells that accumulate in primary and secondary responses and determine the frequency of T cells that are generated (Croft 2010). Mutations affecting CD134 signaling has been linked with Kaposi sarcoma as well as susceptibility to infections.
This clone Ber-ACT35 has been used in flow cytometry experiments to measure D134 expression levels on tumor-infiltrating Treg cells in mouse and human samples (Vargas et al. 2018).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Primate - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- HTLV 1-transformed HUT-102 cells
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P43489
- Entrez Gene
- TNFRSF4
- GO Terms
- GO:0005031 tumor necrosis factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0030890 positive regulation of B cell proliferation
- GO:0032582 negative regulation of gene-specific transcription
- GO:0042098 T cell proliferation
- GO:0043433 negative regulation of transcription factor activity
- GO:0051024 positive regulation of immunoglobulin secretion
- Regulatory
- For research purposes only
Applications of CD134 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | ||
ELISA | |||
Flow Cytometry | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Western Blotting | |||
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the undiluted reagent to label 1x106 cells in 100ul
- Flow Cytometry
- Use 4ul of the undiluted reagent to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the undiluted reagent to label 1x106 cells in 100ul
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:APC | MCA928APC | F | 100 Tests |
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Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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