CD13 antibody | OTI2F10

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Mouse anti CD13

Mouse anti human CD13 antibody recognizes aminopeptidase N, also known as ANPEP, AP-M, AP-N or alanyl aminopeptidase. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI2F10
Isotype
IgG2a
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00367 WB 100 µl
Mouse anti Human CD13 antibody recognizes CD13, also known as ANPEP, AP-M, AP-N, alanyl aminopeptidase, aminopeptidase M, aminopeptidase N, hAPN, microsomal aminopeptidase and myeloid plasma membrane glycoprotein CD13.

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical (provided by RefSeq, Jul 2008). The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in ANPEP appear to be a cause of various types of leukemia or lymphoma.

Mouse anti Human CD13 antibody detects a band of 140-160 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CD13 detects a band of approximately 140-160 kDa in THP-1 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 668-967 of human CD13 (NP_001141) produced in E.coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P15144 Related reagents
Entrez Gene
ANPEP Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0004177 aminopeptidase activity
GO:0008237 metallopeptidase activity
GO:0005829 cytosol
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005793 ER-Golgi intermediate compartment
GO:0008270 zinc ion binding
GO:0030154 cell differentiation
GO:0044419 interspecies interaction between organisms
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD13 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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