CD127 antibody | AbD11590

Human anti Human CD127

Product Type
Monoclonal Antibody
Clone
AbD11590
Isotype
HuCAL Fab bivalent
Specificity
CD127

Product Code Applications Pack Size List Price Your Price Qty
HCA144
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SDS Safety Datasheet SDS
C F * 0.1 mg loader
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loader
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Human anti Human CD127 antibody, clone AbD11590 recognizes the alpha chain of the human interleukin-7 receptor (IL-7R), which is also known as CD127. CD127 is a ~65-75 kDa transmembrane glycoprotein that associates with the interleukin-2 receptor gamma chain to form the functional high affinity IL-7 receptor. CD127 is expressed on lymphoid precursors in the bone marrow, pro-B cells, a subpopulation of thymocytes, T-cells and monocytes. CD127 is the specific receptor for IL-7 and the IL-7 receptor plays a critical role in lymphoid development.

Target Species
Human
Product Form
A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E.coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a DYKDDDDK tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
StrepTactin affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Recombinant human IL-7 R alpha/CD127/Fc chimera
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/10 1/200
Immunohistology - Frozen
Western Blotting
  1. 1HCA144 has been demonstrated to function in Flow Cytometry.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Histology Positive Control Tissue
Human tonsil.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti DYKDDDDK Tag:HRP AHP1074P E WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit anti DYKDDDDK Tag:HRP
Mouse anti Strep-Tag Immo MCA2488 E 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Strep-Tag Immo

Synonyms
IL-7R
RRID
AB_10612595
UniProt
P16871
Entrez Gene
IL7R
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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