CD11b antibody | ICRF44
FITC conjugated Mouse anti Human CD11b antibody, clone ICRF44 (MCA551F) used to evaluate CD11b expression levels on human monocytes and neutrophils by flow cytometry.
Image caption:
Effect of flexible ureterenoscopy on CD11b cell surface expression of neutrophils and monocytes. The points represent median ± Iqr (neutrophils) and mean ± SD (monocytes)
From: Hughes SF, Moyes AJ, Lamb RM, Ella-Tongwiis P, Snyper NYF, Shergill I.
The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.
Eur J Med Res. 2020 Dec 11;25(1):68.
doi: 10.1186/s40001-020-00466-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD11b:Pacific Blue®
- Product Type
- Monoclonal Antibody
- Clone
- ICRF44
- Isotype
- IgG1
- Specificity
- CD11b
Antibody Quality and Performance Guarantee
| Mouse anti Human CD11b antibody, clone ICRF44 recognizes the human CD11b cell surface glycoprotein, a 165 kDa molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes. Mouse anti Human CD11b antibody, clone ICRF44 has been reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cat Cynomolgus monkey Baboon Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Rheumatoid synovial cells and human monocytes.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Pacific Blue® 410 455 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Pacific Blue® | MCA928PB | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Pacific Blue® | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for CD11b antibody
-
Malhotra, V. et al. (1986) Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3).
Eur J Immunol. 16 (9): 1117-23. -
Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocyte.
Leucocyte Typing IV. Oxford University Press 1058-1063. -
Dransfield, I. et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function.
J Cell Biol. 116 (6): 1527-35. -
Rezzonico, R. et al. (2000) Engagement of CD11b and CD11c beta2 integrin by antibodies or soluble CD23 induces IL-1beta production on primary human monocytes through mitogen-activated protein kinase-dependent pathways.
Blood. 95 (12): 3868-77. -
Stirling, R.G. et al. (2001) Interleukin-5 induces CD34(+) eosinophil progenitor mobilization and eosinophil CCR3 expression in asthma.
Am J Respir Crit Care Med. 164: 1403-9. -
Canalli, A.A. et al. (2001) Participation of Mac-1, LFA-1 and VLA-4 integrins in the in vitro adhesion of sickle cell disease neutrophils to endothelial layers, and reversal of adhesion by simvastatin.
Haematologica 96: 526-33. -
Rezzonico, R. et al. (2001) Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB.
Blood. 97 (10): 2932-40. -
Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.
Clin Exp Immunol. 130: 256-62.
View The Latest Product References
-
Glasow, A. et al. (2005) Retinoids and myelomonocytic growth factors co-operatively activate RAR{alpha} and induce human myeloid leukemia cell differentiation via MAP kinase pathways.
Blood 105: 341-9. -
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther. 321: 656-62. -
Patel, S. et al. (2009) Reconstituted high-density lipoprotein increases plasma high-density lipoprotein anti-inflammatory properties and cholesterol efflux capacity in patients with type 2 diabetes.
J Am Coll Cardiol. 53: 962-71. -
Ramacciotti, E. et al. (2011) Evaluation of soluble p-selectin as a marker for the diagnosis of deep venous thrombosis.
Clin Appl Thromb Hemost. 17: 425-31. -
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One 7: e35577. -
Gomes-Alves, P. et al. (2016) In vitro expansion of human cardiac progenitor cells: exploring 'omics tools for characterization of cell-based allogeneic products.
Transl Res. 171: 96-110.e1-3. -
Chen, Y.C. et al. (2018) Effects of normoxic and hypoxic exercise training on the bactericidal capacity and subsequent apoptosis of neutrophils in sedentary men.
Eur J Appl Physiol. 118 (9): 1985-1995. -
Nie, R. et al. (2019) Porphyromonas gingivalis Infection Induces Amyloid-β Accumulation in Monocytes/Macrophages.
J Alzheimers Dis. 72 (2): 479-94. -
Hughes, S.F. et al. (2020) The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.
Eur J Med Res. 25 (1): 68.
- Synonyms
- Integrin Alpha M Chain
- MAC-1
- UniProt
- P11215
- Entrez Gene
- ITGAM
- GO Terms
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
MCA551PB
1611
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