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CD11b antibody | ICRF44

Mouse anti Human CD11b:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
ICRF44
Isotype
IgG1
Specificity
CD11b

Product Code Applications Pack Size List Price Your Price Qty
MCA551A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Mouse anti Human CD11b antibody, clone ICRF44 recognizes the human CD11b cell surface glycoprotein, a 165 kDa molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes.

Mouse anti Human CD11b antibody, clone ICRF44 has been reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Cynomolgus monkey
Baboon
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Rheumatoid synovial cells and human monocytes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
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List Price Your Price
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Description Mouse IgG1 Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD11b antibody

  1. Malhotra, V. et al. (1986) Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3).
    Eur J Immunol. 16 (9): 1117-23.
  2. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocyte.
    Leucocyte Typing IV. Oxford University Press 1058-1063.
  3. Dransfield, I. et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function.
    J Cell Biol. 116 (6): 1527-35.
  4. Rezzonico, R. et al. (2000) Engagement of CD11b and CD11c beta2 integrin by antibodies or soluble CD23 induces IL-1beta production on primary human monocytes through mitogen-activated protein kinase-dependent pathways.
    Blood. 95 (12): 3868-77.
  5. Stirling, R.G. et al. (2001) Interleukin-5 induces CD34(+) eosinophil progenitor mobilization and eosinophil CCR3 expression in asthma.
    Am J Respir Crit Care Med. 164: 1403-9.
  6. Canalli, A.A. et al. (2001) Participation of Mac-1, LFA-1 and VLA-4 integrins in the in vitro adhesion of sickle cell disease neutrophils to endothelial layers, and reversal of adhesion by simvastatin.
    Haematologica 96: 526-33.
  7. Rezzonico, R. et al. (2001) Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB.
    Blood. 97 (10): 2932-40.
  8. Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.
    Clin Exp Immunol. 130: 256-62.
  9. View The Latest Product References
  10. Glasow, A. et al. (2005) Retinoids and myelomonocytic growth factors co-operatively activate RAR{alpha} and induce human myeloid leukemia cell differentiation via MAP kinase pathways.
    Blood 105: 341-9.
  11. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
    J Pharmacol Exp Ther. 321: 656-62.
  12. Patel, S. et al. (2009) Reconstituted high-density lipoprotein increases plasma high-density lipoprotein anti-inflammatory properties and cholesterol efflux capacity in patients with type 2 diabetes.
    J Am Coll Cardiol. 53: 962-71.
  13. Ramacciotti, E. et al. (2011) Evaluation of soluble p-selectin as a marker for the diagnosis of deep venous thrombosis.
    Clin Appl Thromb Hemost. 17: 425-31.
  14. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One 7: e35577.
  15. Gomes-Alves, P. et al. (2016) In vitro expansion of human cardiac progenitor cells: exploring 'omics tools for characterization of cell-based allogeneic products.
    Transl Res. 171: 96-110.e1-3.
  16. Hughes, S.F. et al. (2020) The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.
    Eur J Med Res. 25 (1): 68.

Flow Cytometry

Functional Assays

Synonyms
Integrin Alpha M Chain
MAC-1
RRID
AB_324800
UniProt
P11215
Entrez Gene
ITGAM
GO Terms
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway

MCA551A647

1611

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