CD11b antibody | 2D11

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Mouse anti CD11b

Mouse anti-human CD11b antibody recognizes integrin alpha-M, also known as CR-3 alpha chain, antigen CD11b (p170), cell surface glycoprotein MAC-1 subunit alpha, integrin alpha-M, or leukocyte adhesion receptor MO1.

Product Type
PrecisionAb™ Monoclonal
Clone
2D11
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00481 WB 100 µl
Mouse anti Human CD11b antibody recognizes CD11b also known as integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, cell surface glycoprotein MAC-1 subunit alpha, leukocyte adhesion receptor MO1, macrophage antigen alpha polypeptide or neutrophil adherence receptor alpha-M subunit.

The ITGAM gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for ITGAM (provided by RefSeq, Mar 2009).

Mouse anti Human CD11b antibody detects a band of 170 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CD11b detects a band of approximately 170 kDa in TF-1 cell lysate
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant protein of human CD11b produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P11215 Related reagents
Entrez Gene
ITGAM Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD11b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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