CD114 antibody | LMM775

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Mouse anti Human CD114:RPE

Product Type
Monoclonal Antibody
Clone
LMM775
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1544PET F 25 Tests
MCA1544PE F 100 Tests
Mouse anti Human CD114 antibody, clone LMM775 recognizes the human granulocyte colony-stimulating factor receptor (G-CSF-R) also known as CD114. CD114 is a 836 amino acid, single pass type I transmembrane glycoprotein, a crucial factor in the survival and maturation of cells in the neutrophilic lineage. CD114 contains a single Ig-like C2-type and five fibronectin type-III domains (UniProt: Q99062).

Mouse anti Human CD114 antibody, clone LMM775 binds to an epitope located within the Ig-like domain but does not block binding of G-CSF to its receptor (Leyton et al. 2001). Mouse anti Human CD114 antibody, clone LMM775 does not bind to a mutant G-CSF-R when the Ig-like domain has been substituted with the related gp130 Ig-like domain (Layton et al. 1999). G-CSF stimulates the production of neutrophils and accelerates their maturation in bone marrow (Demetri and Griffin 1991). Expression of CD114 is down regulated on granulocytes but not monocytes following acute exposure to bacterial components including LPS with potential consequences for granulocyte function in the acute response to infection (Dekkers et al. 2000). The G-CSF-receptor on monocytes is functional, playing a role in the release of cytokines, either directly or indirectly following bacterial challange (Boneburg et al. 2000). The G-CSF receptor CD114 has also been implicated in the induction of β-1 integrin mediated adhesion and invasion of pancreatic cancer cells (Chakraborty et al. 2006).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose

Storage Information

Storage
Pack Size: 25 Tests
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
Q99062 Related reagents
Entrez Gene
CSF3R Related reagents
GO Terms
GO:0006952 defense response
GO:0007155 cell adhesion
GO:0005576 extracellular region
GO:0004896 cytokine receptor activity
GO:0005887 integral to plasma membrane
Regulatory
For research purposes only

Applications of CD114 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD114 antibody

  1. Dekkers, P.E. et al. (2000) Granulocyte colony-stimulating factor receptors on granulocytes are down-regulated after endotoxin administration to healthy humans.
    J Infect Dis. 181: 2067-70.
  2. Boneberg, E.M. et al. (2000) Human monocytes express functional receptors for granulocyte colony-stimulating factor that mediate suppression of monokines and interferon-gamma.
    Blood. 95: 270-6.
  3. Layton, J.E. et al. (2001) Identification of ligand-binding site III on the immunoglobulin-like domain of the granulocyte colony-stimulating factor receptor.
    J Biol Chem. 276: 36779-87.
  4. Layton, J.E. et al. (1999) Interaction of granulocyte colony-stimulating factor (G-CSF) with its receptor. Evidence that Glu19 of G-CSF interacts with Arg288 of the receptor.
    J Biol Chem. 274: 17445-51.
  5. Anderson, G. et al. (2006) Thalidomide derivative CC-4047 inhibits osteoclast formation by down-regulation of PU.1.
    Blood. 107: 3098-105.
  6. Chakraborty, A. et al. (2006) Granulocyte Colony-Stimulating Factor Receptor Promotes b1-Integrin–Mediated Adhesion and Invasion of Pancreatic Cancer Cells.
    In: Conference Abstracts From the 2006 Annual Meeting of the International Society of Gastrointestinal Oncology: Session II: Pancreatic cancer. PGCR 1:1, 2006 (ABSTRACT 208)
  7. Layton, J.E. et al. (1997) Neutralising antibodies to the granulocyte colony-stimulating factor receptor recognise both the immunoglobulin-like domain and the cytokine receptor homologous domain.
    Growth Factors.14: 117-30.
  8. Diaz-Romero, J. et al. (2005) Immunophenotypic analysis of human articular chondrocytes: changes in surface markers associated with cell expansion in monolayer culture.
    J Cell Physiol. 202: 731-42.

Further Reading

  1. Nicholson, S.E. et al. (1994) Tyrosine kinase JAK1 is associated with the granulocyte-colony-stimulating factor receptor and both become tyrosine-phosphorylated after receptor activation.
    Proc Natl Acad Sci U S A. 91 (8): 2985-8.