CD107b antibody | H4B4

Mouse anti Human CD107b:FITC

Product Type
Monoclonal Antibody
Clone
H4B4
Isotype
IgG1
Specificity
CD107b

Product Code Applications Pack Size List Price Your Price Qty
MCA6114F
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SDS Safety Datasheet SDS
F 100 Tests/400µl loader
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Mouse anti Human CD107b, clone H4B4 recognizes CD107b. CD107b (also known as LAMP-2) is a major component in lysosomal membranes. It is composed of a polypeptide core of approximately 45 kDa but normally migrates between 100-120 kDa due to high levels of glycosylation (Fukuda, M. et al, 1988).

Clone H4B4 has been reported to suitable for use in Transmission Electron Microscopy (Mane, S. et al, 1988).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Human PBMCs
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 4ul of the undiluted reagent to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

References for CD107b antibody

  1. Fukuda, M. et al. (1988) Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences.
    J Biol Chem. 263 (35): 18920-8.
  2. Mane, S.M. et al. (1989) Purification and characterization of human lysosomal membrane glycoproteins.
    Arch Biochem Biophys. 268 (1): 360-78.

Synonyms
LAMP-2
UniProt
P13473
Entrez Gene
LAMP2
GO Terms
GO:0005886 plasma membrane
GO:0002576 platelet degranulation
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005765 lysosomal membrane
GO:0005770 late endosome
GO:0010008 endosome membrane
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
View more products with CD107B specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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