CD106 antibody | STA

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Mouse anti Human CD106

Mouse anti Human CD106:RPE

Product Type
Monoclonal Antibody
Clone
STA
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2237 F IP 0.2 mg
MCA2237PE F 100 Tests
Mouse anti Human CD106 antibody, clone STA recognizes human CD106, a 110 kDa cell surface protein predominantly expressed on activated vascular endothelium. CD106, also known as vascular cell adhesion molecule 1 (VCAM-1), is also expressed by several non-endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.

CD106 binds the integrins alpha4 beta1 and alpha4 beta7 and mediates leucocyte-endothelial cell adhesion.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Cells of the DS6 T-cell line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P19320 Related reagents
Entrez Gene
VCAM1 Related reagents
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0005902 microvillus
GO:0009897 external side of plasma membrane
GO:0022614 membrane to membrane docking
GO:0030175 filopodium
GO:0030183 B cell differentiation
GO:0042102 positive regulation of T cell proliferation
GO:0044419 interspecies interaction between organisms
GO:0045177 apical part of cell
GO:0050776 regulation of immune response
GO:0050901 leukocyte tethering or rolling
GO:0060333 interferon-gamma-mediated signaling pathway
Regulatory
For research purposes only

Applications of CD106 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunoprecipitation
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD106 antibody

  1. Leca, G. et al. (1995) Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform.
    J Immunol. 154 (3): 1069-77.
  2. David, V. et al. (1990) Identification of a novel 110-kilodalton structure expressed on a subset of T cell receptor-gamma delta-bearing cloned lymphocytes.
    J Immunol. 144: 1-6.