CD106 antibody | STA
Mouse anti Human CD106 antibody, clone STA recognizes human CD106, a ~110 kDa cell surface protein predominantly expressed on activated vascular endothelium. CD106, also known as vascular cell adhesion molecule 1 (VCAM-1), is also expressed by several non-endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.
CD106 binds the integrins alpha4 beta1 and alpha4 beta7 and mediates leucocyte-endothelial cell adhesion.
CD106 binds the integrins alpha4 beta1 and alpha4 beta7 and mediates leucocyte-endothelial cell adhesion.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Cells of the DS6 T-cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NS-1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19320
- Entrez Gene
- VCAM1
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0016021 integral to membrane
- GO:0050839 cell adhesion molecule binding
- GO:0005178 integrin binding
- GO:0005615 extracellular space
- GO:0005902 microvillus
- GO:0009897 external side of plasma membrane
- GO:0022614 membrane to membrane docking
- GO:0030175 filopodium
- GO:0030183 B cell differentiation
- GO:0042102 positive regulation of T cell proliferation
- GO:0044419 interspecies interaction between organisms
- GO:0045177 apical part of cell
- GO:0050776 regulation of immune response
- GO:0050901 leukocyte tethering or rolling
- GO:0060333 interferon-gamma-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of CD106 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/100 | |
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/5 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
Source Reference
-
David, V. et al. (1990) Identification of a novel 110-kilodalton structure expressed on a subset of T cell receptor-gamma delta-bearing cloned lymphocytes.
J Immunol. 144: 1-6.
References for CD106 antibody
-
Leca, G. et al. (1995) Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform.
J Immunol. 154 (3): 1069-77. -
Perdomo-Arciniegas, A-M. & Vernot, J-P. (2011) Co-culture of hematopoietic stem cells with mesenchymal stem cells increases VCAM-1-dependent migration of primitive hematopoietic stem cells.
Int J Hematol. 94 (6): 525-32. -
van Beijnum, J.R. et al. (2008) Angiogenic profiling and comparison of immortalized endothelial cells for functional genomics.
Exp Cell Res. 314 (2): 264-72.
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