CD106 antibody | 3H10

100% Secure

Anti CD106 Antibody, clone 3H10 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CD106 antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 7 second exposure. Arrow points to CD106 (molecular weight 99 kDa)

Mouse anti CD106

Mouse anti-human VCAM1 antibody recognizes vascular cell adhesion protein 1, also known as CD106 antigen. This antibody has been extensively validated for western blotting using whole cell lysates.

CD106 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: 3H10
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00461	WB	100 µl
VMA00461KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human CD106 antibody recognizes CD106, also known as VCAM1 or vascular cell adhesion protein 1.

VCAM1 gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for VCAM1 (provided by RefSeq, Dec 2010).

Mouse anti Human CD106 antibody detects a band of 99 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti CD106 detects a band of approximately 99 kDa in HEK293 cell lysate

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from ascites

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length recombinant protein of human CD106 (VCAM1) produced in HEK293T cells

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P19320 Related reagents
Entrez Gene: VCAM1 Related reagents
GO Terms: GO:0007157 heterophilic cell-cell adhesion; GO:0016021 integral to membrane; GO:0050839 cell adhesion molecule binding; GO:0005178 integrin binding; GO:0005615 extracellular space; GO:0005902 microvillus; GO:0009897 external side of plasma membrane; GO:0022614 membrane to membrane docking; GO:0030175 filopodium; GO:0030183 B cell differentiation; GO:0042102 positive regulation of T cell proliferation; GO:0044419 interspecies interaction between organisms; GO:0045177 apical part of cell; GO:0050776 regulation of immune response; GO:0050901 leukocyte tethering or rolling; GO:0060333 interferon-gamma-mediated signaling pathway
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of CD106 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information

Online Payment Methods:

Invoice / Purchase Order
Credit Card (Maestro, VISA,MasterCard & VISA Electron)

View more products with CD106 specificity