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CD106 antibody | 3H10

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Western Blot Validated
Anti CD106 Antibody, clone 3H10 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with CD106 antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 7 second exposure. Arrow points to CD106 (molecular weight 99 kDa)

Mouse anti CD106

Product Type
PrecisionAb Monoclonal
Clone
3H10
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00461
Datasheet
WB
SDS
100 µl loader

Mouse anti Human CD106 antibody recognizes CD106, also known as VCAM1 or vascular cell adhesion protein 1.

VCAM1 gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for VCAM1 (provided by RefSeq, Dec 2010).

Mouse anti Human CD106 antibody detects a band of 99 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CD106 detects a band of approximately 99 kDa in HEK293 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant protein of human CD106 (VCAM1) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P19320
Entrez Gene
VCAM1
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0005902 microvillus
GO:0009897 external side of plasma membrane
GO:0022614 membrane to membrane docking
GO:0030175 filopodium
GO:0030183 B cell differentiation
GO:0042102 positive regulation of T cell proliferation
GO:0044419 interspecies interaction between organisms
GO:0045177 apical part of cell
GO:0050776 regulation of immune response
GO:0050901 leukocyte tethering or rolling
GO:0060333 interferon-gamma-mediated signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD106 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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