CD106 antibody | 3H10
Mouse anti CD106
Mouse anti-human VCAM1 antibody recognizes vascular cell adhesion protein 1, also known as CD106 antigen. This antibody has been extensively validated for western blotting using whole cell lysates.
CD106 Antibody with Control Lysate
Mouse anti-human VCAM1 antibody recognizes vascular cell adhesion protein 1, also known as CD106 antigen. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- 3H10
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00461 | WB | 100 µl | ||||
| VMA00461KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human CD106 antibody recognizes CD106, also known as VCAM1 or vascular cell adhesion protein 1.
VCAM1 gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for VCAM1 (provided by RefSeq, Dec 2010).
Mouse anti Human CD106 antibody detects a band of 99 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
VCAM1 gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for VCAM1 (provided by RefSeq, Dec 2010).
Mouse anti Human CD106 antibody detects a band of 99 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CD106 detects a band of approximately 99 kDa in HEK293 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant protein of human CD106 (VCAM1) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P19320 Related reagents
- Entrez Gene
- VCAM1 Related reagents
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0016021 integral to membrane
- GO:0050839 cell adhesion molecule binding
- GO:0005178 integrin binding
- GO:0005615 extracellular space
- GO:0005902 microvillus
- GO:0009897 external side of plasma membrane
- GO:0022614 membrane to membrane docking
- GO:0030175 filopodium
- GO:0030183 B cell differentiation
- GO:0042102 positive regulation of T cell proliferation
- GO:0044419 interspecies interaction between organisms
- GO:0045177 apical part of cell
- GO:0050776 regulation of immune response
- GO:0050901 leukocyte tethering or rolling
- GO:0060333 interferon-gamma-mediated signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of CD106 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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