CD102 antibody | CBRIC2/2
Mouse anti Human CD102:Alexa Fluor® 647
Mouse anti Human CD102
- Product Type
- Monoclonal Antibody
- Clone
- CBRIC2/2
- Isotype
- IgG2a
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2290A647 | F | 100 Tests/1ml |
|
|
| MCA2290A647T | F | 25 Tests/0.25ml |
|
|
| MCA2290 | C F IP | 0.2 mg |
|
Mouse anti Human CD102 antibody, clone CBRIC2/2 recognizes human CD102, also known as intercellular adhesion molecule-2 (ICAM-2). CD102 is a glycosylated cell surface protein that is broadly expressed on most leucocytes and is strongly expressed by endothelial cells. CD102 interacts with the CD11a/CD18 (LFA-1). CD102 may play a role in lymphocyte re-circulation and T-cell activation (Yamashita et al. 2008).
Mouse anti Human CD102 antibody, clone CBRIC2/2 is reported to inhibit interactions between CD102 and LFA-1 (De Fougerolles et al. 1991).
Mouse anti Human CD102 antibody, clone CBRIC2/2 is reported to inhibit interactions between CD102 and LFA-1 (De Fougerolles et al. 1991).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Transfected COS cells expressing ICAM-2 cDNA.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised BALB/c mice were fused with cells of the Murine P3X63Ag.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P13598
- Entrez Gene
- ICAM2
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005178 integrin binding
- GO:0016337 cell-cell adhesion
- GO:0050776 regulation of immune response
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD102 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/100 | ||
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA929A647 | F | 100 Tests/1ml |
|
|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
Source Reference
-
de Fougerolles, A.R. et al. (1991) Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1.
J Exp Med. 174 (1): 253-67.
References for CD102 antibody
-
Xu, H. et al. (1996) Characterization of murine intercellular adhesion molecule-2.
J Immunol. 156 (12): 4909-14. -
Diacovo, T.G. et al. (1994) A functional integrin ligand on the surface of platelets: intercellular adhesion molecule-2.
J Clin Invest. 94 (3): 1243-51. -
Porter, J.C. & Hall, A. (2009) Epithelial ICAM-1 and ICAM-2 regulate the egression of human T cells across the bronchial epithelium.
FASEB J. 23 (2): 492-502. -
Banerjee, P. et al. (2007) Human T-cell leukemia virus type 1 (HTLV-1) p12I down-modulates ICAM-1 and -2 and reduces adherence of natural killer cells, thereby protecting HTLV-1-infected primary CD4+ T cells from autologous natural killer cell-mediated cytotoxicity despite the reduction of major histocompatibility complex class I molecules on infected cells.
J Virol. 81: 9707-17. -
Arjmandi A et al. (2009) Dendritic cell adhesion to cerebral endothelium: role of endothelial cell adhesion molecules and their ligands.
J Neuropathol Exp Neurol. 68 (3): 300-13.
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