CD102 antibody | CBRIC2/2

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Anti Human CD102 Antibody, clone CBRIC2/2 thumbnail image 1

Anti Human CD102 Antibody, clone CBRIC2/2 thumbnail image 2

Anti Human CD102 Antibody, clone CBRIC2/2 gallery image 1 — Figure A. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse anti Human CD102 (MCA2290PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Anti Human CD102 Antibody, clone CBRIC2/2 gallery image 2 — Figure A. Alexa Fluor 700 conjugated mouse anti human CD3 (MCA463A700) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 700 conjugated mouse anti human CD3 (MCA463A700) and FITC conjugated mouse anti human CD102 (MCA2290F). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of human seroblock (BUF070A). Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Human CD102:Alexa Fluor® 647

Mouse anti Human CD102:FITC

Mouse anti Human CD102

Product Type: Monoclonal Antibody
Clone: CBRIC2/2
Isotype: IgG2a

Product Code	Applications	Pack Size
MCA2290A647T	F	25 Tests/0.25ml
MCA2290A647	F	100 Tests/1ml
MCA2290F	F	0.1 mg
MCA2290	C F IP	0.2 mg

Mouse anti Human CD102 antibody, clone CBRIC2/2 recognizes human CD102, also known as intercellular adhesion molecule-2 (ICAM-2). CD102 is a glycosylated cell surface protein that is broadly expressed on most leucocytes and is strongly expressed by endothelial cells. CD102 interacts with the CD11a/CD18 (LFA-1). Reports suggest that the CD102 may play a role in lymphocyte re-circulation and T-cell activation.

Mouse anti Human CD102 antibody, clone CBRIC2/2 is reported to inhibit interactions between CD102 and LFA-1 (De Fougerolles et al. 1991).

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Alexa Fluor® 647- liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Transfected COS cells expressing ICAM-2 cDNA.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Cells from immunised BALB/c mice were fused with cells of the Murine P3X63Ag.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P13598 Related reagents
Entrez Gene: ICAM2 Related reagents
GO Terms: GO:0005887 integral to plasma membrane; GO:0005178 integrin binding; GO:0016337 cell-cell adhesion; GO:0050776 regulation of immune response
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD102 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry	Neat	1/10
Flow Cytometry		1/100
Immunohistology - Frozen
Immunoprecipitation

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG2a Negative Control:Alexa Fluor® 647	MCA929A647	100 Tests/1ml	F
Mouse IgG2a Negative Control:FITC	MCA929F	100 Tests	F
Mouse IgG2a Negative Control	MCA929	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Product Specific References

Source Reference

de Fougerolles, A.R. et al. (1991) Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1.
J Exp Med. 174 (1): 253-67.

References for CD102 antibody

Xu, H. et al. (1996) Characterization of murine intercellular adhesion molecule-2.
J Immunol. 156 (12): 4909-14.
Diacovo, T.G. et al. (1994) A functional integrin ligand on the surface of platelets: intercellular adhesion molecule-2.
J Clin Invest. 94 (3): 1243-51.
Porter, J.C. & Hall, A. (2009) Epithelial ICAM-1 and ICAM-2 regulate the egression of human T cells across the bronchial epithelium.
FASEB J. 23 (2): 492-502.
Banerjee, P. et al. (2007) Human T-cell leukemia virus type 1 (HTLV-1) p12I down-modulates ICAM-1 and -2 and reduces adherence of natural killer cells, thereby protecting HTLV-1-infected primary CD4+ T cells from autologous natural killer cell-mediated cytotoxicity despite the reduction of major histocompatibility complex class I molecules on infected cells.
J Virol. 81: 9707-17.
Arjmandi A et al. (2009) Dendritic cell adhesion to cerebral endothelium: role of endothelial cell adhesion molecules and their ligands.
J Neuropathol Exp Neurol. 68 (3): 300-13.

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