CD10 antibody | OTI2A3

100% Secure


Mouse anti CD10

Product Type
PrecisionAb Monoclonal
Clone
OTI2A3
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00717
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human CD10 antibody recognizes CD10, also known as atriopeptidase, neprilysin, CALLA and skin fibroblast elastase (SFE).

MME encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. MME, which encodes a 100 kDa type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of MME is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing (provided by RefSeq, Jul 2008).

Mouse anti Human CD10 antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti CD10 detects a band of approximately 110 kDa in LNCaP cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
E. coli-derived human recombinant protein of amino acids 411-750 of human CD10

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
P08473 Related reagents
Entrez Gene
MME Related reagents
GO Terms
GO:0005515 protein binding
GO:0004222 metalloendopeptidase activity
GO:0005887 integral to plasma membrane
GO:0046872 metal ion binding
GO:0006508 proteolysis
GO:0007267 cell-cell signaling
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Western Blotting without Trial and Error: Use PrecisionAb Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.