CCT8 antibody | AB01/1C4

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Mouse anti CCT8

Product Type
PrecisionAb Monoclonal
Clone
AB01/1C4
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00881
Datasheet
WB
SDS
100 µl loader

Mouse anti CCT8 antibody recognizes chaperonin containing T-complex polypeptide 1 subunit 8, also known as CCT-theta.

CCT8 is a component of the chaperonin-containing T-complex (TRiC), also called CCT, a large double-ring complex that appears to have the primary function of participating in protein folding (Freund et al. 2014). Each TriC subunit, named CCT1-8, is found at a fixed position within the ring. In eukaryotes, TRiC supports folding of ~10% of the cytosolic proteome, and is required for folding of the cytoskeletal protein actin (Balchin et al. 2018). Many products of genes that are deregulated in cancers are client proteins of TRiC, which suggests a role of TRiC in cancer development (Carr et al. 2017). Upregulation of CCT8 has been identified in hepatocellular carcinoma (HCC) cells, and plays an important role in their proliferation (Huang et al. 2014). CCT8 has also been suggested as a predictive marker for pancreatic cancer (Liu et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti CCT8 antibody detects a band of approximately 56 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-548 of human CCT8
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P50990
Entrez Gene
CCT8
GO Terms
GO:0051082 unfolded protein binding
GO:0042623 ATPase activity, coupled
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0005815 microtubule organizing center
GO:0016235 aggresome
GO:0045111 intermediate filament cytoskeleton
GO:0051084 'de novo' posttranslational protein folding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of CCT8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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