CCT Beta antibody

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Rat anti CCT Beta

CCT beta antibody recognizes T-complex protein 1 subunit beta, also known as TCP-1-beta or CCT-beta. The antibody has been extensively validated for western blotting using whole cell lysates.

CCT Beta Antibody with Control Lysate

CCT beta antibody recognizes T-complex protein 1 subunit beta, also known as TCP-1-beta or CCT-beta. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00026 WB 100 µl
VMA00026KT WB 2 Westerns
With Lysate 400µg
Rat anti Human CCT beta antibody recognizes human T-complex protein 1 subunit beta, also known as CCT beta, CCT2 or TCP-1-beta. CCT beta is a 535 amino acid cytoplasmically expressed chaperonin molecule involved in protein folding and has been shown to be involved in actin interaction (Llorca et al. 1999). Two isoforms of CCT beta have been described, arising from alternative splicing. It is not clear whether this Rat anti Human CCT beta antibody recognizes the shorter isoform 2, lacking the N-terminal 47 amino acids seen in isoform 1.

Rat anti Human CCT beta antibody recognizes CCT beta as a single band of ~ 57 kDa in multiple human cell line lysates by western blotting under reducing conditions and is cross reactive with both mouse and rat cell line lysates.

Product Details

Target Species
Human
Western Blotting
Anti CCT beta antibody detects a band of approximately 57 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Mouse CCT beta peptide conjugated to purified tuberculin protein derivative
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P78371 Related reagents
Entrez Gene
CCT2 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0051084 'de novo' posttranslational protein folding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CCT Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P

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