CCT Beta antibody | PK/8/4/4i/2F
Rat anti CCT beta antibody, clone PK/8/4/4i/2F used for the detection of CCTβ in fetal and adult rabbit fibroblast lysates by western blotting.
Image caption:
CCT-eta but not CCT-beta protein and mRNA are differentially expressed in fetal versus adult fibroblasts. RNA and protein extracted from fetal and adult fibroblasts were subjected to qRT PCR (a & b) and Western blot (c & d) analyses respectively. CCT-eta mRNA was significantly more abundant in adult fibroblasts when compared to fetal fibroblasts (a); there was no significant difference in CCT-beta message levels between fetal and adult fibroblasts (b). Values are means ± SEM of three independent studies performed in duplicate. Statistical analyses were performed using Student's t test. NS = non-significant. Equal amounts of protein loaded from fetal and adult fibroblasts showed that adult fibroblasts express significantly greater CCT-eta protein(c). In contrast CCT-beta protein levels were not different between fetal and adult fibroblasts (d). Blots shown here are representative of at least three different experiments.
From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility.
PLoS ONE 5(4): e10063.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti CCT beta antibody, clone PK/8/4/4i/2F used for the detection of CCTβ in fetal and adult rabbit fibroblast lysates by western blotting.
Image caption:
siRNAs against CCT-eta and CCT-beta decrease both basal and EGF- induced mRNA and protein levels of their targets in fibroblasts. (a & b) qRT-PCR analysis of CCT-eta and CCT-beta mRNA levels showed effective inhibition of both basal expression and EGF-induction in siRNA-transfected adult fibroblasts. Results are expressed as relative quotient (RQ) of measured CCT-eta or CCT-beta mRNA and were calculated as a percentage of baseline control levels (100%). Values are means ± SEM of six independent studies, each performed in duplicate. Statistical analyses were performed with Student's t test. Ntx- no transfection; EGF-EGF treatment (1 nM); siRNA-treatment with CCT-eta/CCT-beta siRNA; Scr- treatment with scrambled control siRNA. (c & d) Western blot results using CCT-eta and CCT-beta antibody (1:500) showed effective reduction of CCT-eta and CCT-beta protein levels when siRNA was administered but no decrease when scrambled siRNA was employed. GAPDH was used as a loading control. A representative immunoblot of up to four similar such blots is shown for each analysis.
From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility.
PLoS ONE 5(4): e10063.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti CCT beta antibody, clone PK/8/4/4i/2F used for the detection of CCTβ in fetal and adult rabbit fibroblast lysates by western blotting.
Image caption:
siRNA downregulation of CCT-eta but not CCT-beta reduces α-SMA protein levels in adult fibroblasts. (a & b)Western blot showed effective reduction of both CCT-eta and α-SMA protein levels when CCT-eta siRNA was administered, leaving beta-actin largely unaffected. In contrast, downregulating CCT-beta did not lead to significant reduction in either α-SMA or beta-actin levels. GAPDH was used as loading internal control. A representative immunoblot of up to three similar such blots are shown for each analysis. Ntx- no transfection; EGF-EGF treatment (1 nM); siRNA-treatment with CCT-eta/CCT-beta siRNA; Scr- treatment with scrambled control siRNA.
From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility.
PLoS ONE 5(4): e10063.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti CCT beta antibody, clone PK/8/4/4i/2F recognises the beta polypeptide of the CCT chaperonin molecule complex.
The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Bovine Chicken Sheep Mouse Dog Rabbit Hamster Pig Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P78371
- Entrez Gene
- CCT2
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0051084 'de novo' posttranslational protein folding
- Regulatory
- For research purposes only
Applications of CCT Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/100 | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA2275 detects a band of approximately 60kD in lysates of heat shocked HeLa cells.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Product Specific References
References for CCT Beta antibody
-
Satish L et al. (2010) Cloning and expression of rabbit CCT subunits eta and beta in healing cutaneous wounds.
Cell Stress Chaperones. 15 (6): 819-26. -
Fislová, T. et al. (2010) Association of the influenza virus RNA polymerase subunit PB2 with the host chaperonin CCT.
J Virol. 84 (17): 8691-9. -
Satish, L. et al. (2010) Chaperonin containing T-complex polypeptide subunit eta (CCT-eta) is a specific regulator of fibroblast motility and contractility.
PLoS One. 5(4):e10063. -
Plimpton, R.L. et al. (2015) Structures of the Gβ-CCT and PhLP1-Gβ-CCT complexes reveal a mechanism for G-protein β-subunit folding and Gβγ dimer assembly.
Proc Natl Acad Sci U S A. 112 (8): 2413-8. -
Satish, L. et al. (2011) Chaperonin containing T-complex polypeptide (CCT) subunit expression in oral mucosal wounds and fibroblasts.
Cell Stress Chaperones. 16 (6): 675-80.
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