CCL13 antibody | B06-1G9

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Mouse anti Human CCL13

Product Type
Monoclonal Antibody
Clone
B06-1G9
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6176GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human CCL13 antibody, clone B06-1G9, recognizes CCL13, a member of the CC family of cytokines. CCL13, also known as MCP-4, is a 98 amino acid (aa) precursor protein which contains a 23 aa hydrophobic signal peptide that is cleaved to the mature form of the protein which is 75aa protein (8kDa). CCL13 is a chemoattractant for various cells including monocytes, T cells, immature dendritic cells and eosinophils (Entiquez & Garcia-Zepeda 2013). It has also been associated with various inflammatory diseases including asthma, arthritis, atherosclerosis and multiple sclerosis (Procaccini et al. 2015). CCL13 can bind to several chemokine receptors (CCR1, CCR2 and CCR3) to elicit chemoattractant responses. It is also known to induce degranulation of eosinophils and the secretion of pro-inflammatory cytokines.

This purified Mouse anti Human CCL13 antibody, clone B06-1G9 (MCA6176GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human CCL13 antibody, clone B16-3D8 (MCA6177B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human full length CCL13 (Phe17-Thr98)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q99616
Entrez Gene
CCL13
GO Terms
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006874 cellular calcium ion homeostasis
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of CCL13 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human CCL13:Biotin MCA6177B E 0.1 mg loader

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