CCAR2 antibody | D02/1C5-4

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siRNA Validated

Anti CCAR2 Antibody, clone D02/1C5-4 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti CCAR2 Antibody, clone D02/1C5-4 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti CCAR2 Antibody, clone D02/1C5-4 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti CCAR2 Antibody, clone D02/1C5-4 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CCAR2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 31 second exposure. Arrow points to CCAR2 (molecular weight 130 kDa)

Anti CCAR2 Antibody, clone D02/1C5-4 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against CCAR2 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti CCAR2 antibody (**VMA00564**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 60 second (chemiluminescent channel) and 10 second (rhodamine channel) exposure.

Anti CCAR2 Antibody, clone D02/1C5-4 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of CCAR2 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against CCAR2 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti CCAR2

Product Type: PrecisionAb Monoclonal
Clone: D02/1C5-4
Isotype: IgG1
Format: Purified
Specificity: CCAR2

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VMA00564 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human CCAR2 antibody recognizes the cell cycle and apoptosis regulator protein 2, also known as cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1 or p30 DBC protein.

Mouse anti Human CCAR2 antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti CCAR2 detects a band of approximately 130 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human CCAR2 (amino acids 5 - 366)
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.