Cbfb antibody | CD06/4G9
Mouse anti CBFB antibody recognizes core-binding factor subunit beta, also known as PEA2-beta. CBFB is a transcription factor. Along with its binding partner, RUNX1, CBFB regulates a range of signaling pathways to maintain cell and tissue homeostasis. RUNX1 has sequence-specific transcription factor activity, and CBFB enhances the activity of RUNX1. Genetic alterations in CBFB and RUNX1 are linked with a range of human disorders and cancers (Malik et al. 2019). The role of CBFB in bone and cartilage development has been well-characterized, with CBFB regulating the proliferation and differentiation of chondrocytes and osteoblast-lineage cells via multiple signaling pathways (Komori 2020). CBFB is a commonly mutated driver of human cancers, including blood cancer and breast cancer (Malik et al. 2019). Furthermore, CBFB has been identified as one of the six most significantly mutated genes in breast cancer (Sustic et al. 2020).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti CBFB detects a band of approximately 21 kDa in RAW264.7 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-187 of human CBFB
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13951
- Entrez Gene
- CBFB
- GO Terms
- GO:0005515 protein binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003702 RNA polymerase II transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0005634 nucleus
- GO:0006366 transcription from RNA polymerase II promoter
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Cbfb antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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