Cbfb antibody | CD06/4G9

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Mouse anti Cbfb

Product Type
PrecisionAb Monoclonal
Clone
CD06/4G9
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00957
Datasheet
WB
SDS
100 µl loader

Mouse anti CBFB antibody recognizes core-binding factor subunit beta, also known as PEA2-beta. CBFB is a transcription factor. Along with its binding partner, RUNX1, CBFB regulates a range of signaling pathways to maintain cell and tissue homeostasis. RUNX1 has sequence-specific transcription factor activity, and CBFB enhances the activity of RUNX1. Genetic alterations in CBFB and RUNX1 are linked with a range of human disorders and cancers (Malik et al. 2019). The role of CBFB in bone and cartilage development has been well-characterized, with CBFB regulating the proliferation and differentiation of chondrocytes and osteoblast-lineage cells via multiple signaling pathways (Komori 2020). CBFB is a commonly mutated driver of human cancers, including blood cancer and breast cancer (Malik et al. 2019). Furthermore, CBFB has been identified as one of the six most significantly mutated genes in breast cancer (Sustic et al. 2020).

Product Details

Target Species
Human
Western Blotting
Mouse anti CBFB detects a band of approximately 21 kDa in RAW264.7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-187 of human CBFB
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q13951
Entrez Gene
CBFB
GO Terms
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003702 RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0005634 nucleus
GO:0006366 transcription from RNA polymerase II promoter
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Cbfb antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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