Cathepsin D antibody

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Mouse anti Cathepsin D

Cathepsin D Antibody with Control Lysate

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00581 WB 100 µl
VMA00581KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human cathepsin D antibody recognizes cathepsin D, also known as CLN10, CPSD, ceroid-lipofuscinosis neuronal 10, epididymis secretory sperm binding protein Li 130P, or lysosomal aspartyl protease.

CTSD gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of CTSD is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in CTSD are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease (provided by RefSeq, Jul 2008).

Mouse anti Human cathepsin D antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
Buffer Solution
HEPES buffered saline
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
E. coli-derived recombinant human protein cathepsin D

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P07339 Related reagents
Entrez Gene
CTSD Related reagents
GO Terms
GO:0004190 aspartic-type endopeptidase activity
GO:0005764 lysosome
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0008219 cell death
GO:0042470 melanosome
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cathepsin D antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti cathepsin D detects a band of approximately 28 kDa in MCF7 cell lysates
Western Blotting
Anti cathepsin D detects a band of approximately 28 kDa in MCF7 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB