Catenin Beta-Like Protein 1 antibody
Mouse anti Catenin Beta-Like Protein 1
Catenin beta-like protein 1 antibody recognizes catenin beta-like protein 1, also known as nuclear associated protein or testis development protein NYD-SP19. This antibody has been extensively validated for western blotting using whole cell lysates.
Catenin Beta-Like Protein 1 Antibody with Control Lysate
Catenin beta-like protein 1 antibody recognizes catenin beta-like protein 1, also known as nuclear associated protein or testis development protein NYD-SP19. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00238 | WB | 100 µl | ||||
| VMA00238KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human catenin beta-like protein 1 antibody recognizes the beta-catenin-like protein 1, also known as nuclear associated protein and testis development protein NYD-SP19.
Encoded by the CTNNBL1 gene, catenin beta-like protein 1 is a component of the pre-mRNA-processing factor 19-cell division cycle 5-like (PRP19-CDC5L) protein complex, which activates pre-mRNA splicing and is an integral part of the spliceosome. The encoded protein is also a nuclear localization sequence binding protein, and binds to activation-induced deaminase and is important for antibody diversification. CTNNBL1 may also be associated with the development of obesity. Alternative splicing results in multiple transcript variants. A pseudogene of CTNNBL1 has been defined on the X chromosome (provided by RefSeq, Jul 2013).
Mouse anti Human catenin beta-like protein 1 antibody detects a band of 65 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the CTNNBL1 gene, catenin beta-like protein 1 is a component of the pre-mRNA-processing factor 19-cell division cycle 5-like (PRP19-CDC5L) protein complex, which activates pre-mRNA splicing and is an integral part of the spliceosome. The encoded protein is also a nuclear localization sequence binding protein, and binds to activation-induced deaminase and is important for antibody diversification. CTNNBL1 may also be associated with the development of obesity. Alternative splicing results in multiple transcript variants. A pseudogene of CTNNBL1 has been defined on the X chromosome (provided by RefSeq, Jul 2013).
Mouse anti Human catenin beta-like protein 1 antibody detects a band of 65 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti catenin beta-like protein 1 detects a band of approximately 65 kDa in Jurkat cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant protein corresponding to amino acids 390-557 of human catenin beta-like protein 1 expressed in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q8WYA6 Related reagents
- Entrez Gene
- CTNNBL1 Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Catenin Beta-Like Protein 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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