Caspase-3 antibody

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Mouse anti Caspase-3

Mouse anti human caspase-3 antibody recognizes caspase-3, also known as CASP-3, CASP3, CPP-32, PARP cleavage protease, SREBP cleavage activity 1, apopain or protein Yama (protein of death). This antibody has been extensively validated for western blotting using whole cell lysates.

Caspase-3 Antibody with Control Lysate

Mouse anti human caspase-3 antibody recognizes caspase-3, also known as CASP-3, CASP3, CPP-32, PARP cleavage protease, SREBP cleavage activity 1, apopain or protein Yama (protein of death). This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00412 WB 100 µl
VMA00412KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human caspase-3 antibody recognizes caspase-3, also known as CASP-3, CPP-32, PARP cleavage protease, SREBP cleavage activity 1 and protein Yama (protein of death).

Encoded by the CASP3 gene, caspase-3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in apoptosis or programmed cell death. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase-3 cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of CASP3 results in two transcript variants that encode the same protein (provided by RefSeq, Jul 2008).

Mouse anti Human caspase-3 antibody detects a band of 32 kDa corresponding to the full-length active protein. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti caspase-3 detects a band of approximately 32 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E.coli-derived recombinant protein corresponding to aa 27-175 of human caspase-3
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P42574 Related reagents
Entrez Gene
CASP3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0006508 proteolysis
GO:0008624 induction of apoptosis by extracellular signals
GO:0008629 induction of apoptosis by intracellular signals
GO:0008635 activation of caspase activity by cytochrome c
GO:0030264 nuclear fragmentation involved in apoptotic nuclear change
GO:0034612 response to tumor necrosis factor
GO:0043066 negative regulation of apoptosis
GO:0048011 nerve growth factor receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Caspase-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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