Casein Kinase 2 antibody | AbD05928

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Human anti Casein Kinase 2 Alpha 1

Product Type
Monoclonal Antibody
Clone
AbD05928
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA064 E WB 0.1 mg
Human anti casein kinase 2 alpha 1 antibody, clone AbD05928 recognizes human casein kinase 2 alpha 1. Casein kinase II (CK2) is a heterotetrameric serine/threonine protein kinase consisting of two 44 kDa catalytic subunits (CK2-alpha) and two 26 kDa regulatory subunits (CK2-beta). The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. CK2 is constitutively expressed in most cells and tissues and is highly conserved among eukaryotes. It participates in a wide variety of cellular processes including protein kinase networks that control cell cycle progression and cellular stress responses with over 300 substrates. Consequently, fluctuations in the expression of CK II have been linked with various human diseases and most notably, certain cancers (breast, prostate, lung etc).

Product Details

Target Species
Human
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Full length recombinant human CK2-alpha1 expressed in Sf9 cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P68400 Related reagents
Entrez Gene
CSNK2A1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0016055 Wnt receptor signaling pathway
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0007411 axon guidance
GO:0016580 Sin3 complex
GO:0016581 NuRD complex
GO:0047485 protein N-terminus binding
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Casein Kinase 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Western Blotting
HCA064 detects a band of approximately 44 kDa in HCT-116 cell lysates

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