Casein Kinase 2 Alpha 1 antibody

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Human anti Casein Kinase 2 Alpha 1

Casein kinase 2 alpha 1 antibody recognizes casein kinase II subunit alpha, also known as CK II alpha. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00068
Datasheet
WB
MSDS
100 µl loader

Human anti Human casein kinase 2 alpha 1 antibody recognizes the casein kinase 2 subunit alpha, also known as CK II alpha.

Casein kinase 2 alpha 1 is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by CSNK2A1 represents the alpha subunit. While CSNK2A1 is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for CSNK2A1 (provided by RefSeq, Jul 2008).

Human anti Human casein kinase 2 alpha 1 antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti casein kinase 2 alpha 1 detects a band of approximately 42 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a DYKDDDDK tag and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Full length recombinant human casein kinase 2 alpha1 expressed in Sf9 cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P68400 Related reagents
Entrez Gene
CSNK2A1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0016055 Wnt receptor signaling pathway
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0007411 axon guidance
GO:0016580 Sin3 complex
GO:0016581 NuRD complex
GO:0047485 protein N-terminus binding
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of Casein Kinase 2 Alpha 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader
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