Casein Kinase 2 Alpha 1 antibody | AbD22725.1
KO Validated
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WB ResetHuman anti Casein Kinase 2 Alpha 1
- Product Type
- PrecisionAb Monoclonal
- Clone
- AbD22725.1
- Isotype
- HuCAL Fab bivalent
- Format
- Purified
- Specificity
- Casein Kinase 2 Alpha 1
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| Human anti Human casein kinase 2 alpha 1 antibody recognizes the casein kinase 2 subunit alpha, also known as CK II alpha. Casein kinase 2 alpha 1 is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by CSNK2A1 represents the alpha subunit. While CSNK2A1 is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for CSNK2A1 (provided by RefSeq, Jul 2008). Human anti Human casein kinase 2 alpha 1 antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Anti casein kinase 2 alpha 1 detects a band of approximately 42 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a DYKDDDDK tag and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
- Preparation
- Recombinant human monoclonal antibody purified by metal chelate affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- Full length recombinant human casein kinase 2 alpha1 expressed in Sf9 cells
- Approx. Protein Concentrations
- F(ab')2 concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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- UniProt
- P68400
- Entrez Gene
- CSNK2A1
- GO Terms
- GO:0005886 plasma membrane
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0016055 Wnt receptor signaling pathway
- GO:0005524 ATP binding
- GO:0006468 protein phosphorylation
- GO:0007411 axon guidance
- GO:0016580 Sin3 complex
- GO:0016581 NuRD complex
- View More GO Terms
- GO:0047485 protein N-terminus binding
View more products with Casein Kinase 2 Alpha 1 specificity
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