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Carbonic Anhydrase VIII antibody

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Western Blot Validated
Anti Carbonic Anhydrase VIII Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with carbonic anhydrase VIII antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 66.9 second exposure. Arrow points to carbonic anhydrase VIII (molecular weight 33 kDa)

Mouse anti Carbonic Anhydrase VIII

Carbonic anhydrase VIII antibody recognizes carbonic anhydrase-related protein. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified
PrecisionAb Antibodies
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00350 WB 100 µl
Mouse anti Human carbonic anhydrase VIII antibody detects carbonic anhydrase VIII, also known as carbonic anhydrase-related protein. Carbonic anhydrase VIII is a member of a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa).

Mouse anti human carbonic anhydrase VIII antibody has been extensively validated for western blotting using whole cell lysates and detects a band of 33kDa.

Product Details

Target Species
Human
Western Blotting
Anti carbonic anhydrase VIII detects a band of approximately 33 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human carbonic anhydrase VIII
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P35219 Related reagents
Entrez Gene
CA8 Related reagents
GO Terms
GO:0004089 carbonate dehydratase activity
GO:0006730 one-carbon metabolic process
GO:0008270 zinc ion binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Carbonic Anhydrase VIII antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
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