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Carbonic Anhydrase VIII antibody

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Anti Carbonic Anhydrase VIII Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Carbonic Anhydrase VIII Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Carbonic Anhydrase VIII Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti Carbonic Anhydrase VIII Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with carbonic anhydrase VIII antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 66.9 second exposure. Arrow points to carbonic anhydrase VIII (molecular weight 33 kDa)
Anti Carbonic Anhydrase VIII Antibody (PrecisionAb Monoclonal Antibody) gallery image 2
Figure A. StarBright Violet 670 conjugated Mouse anti Human CD45 (MCA87SBV670) and purified Mouse IgG2b isotype control (MCA691) detected with Goat anti Mouse IgG2b FITC (STAR134F). Figure B. StarBright Violet 670 conjugated Mouse anti Human CD45 (MCA87SBV670) and purified Carbonic Anhydrase VIII (VMA00350) detected with Goat anti Mouse IgG2b FITC (STAR134F). All experiments performed on Leucoperm (BUF09) fixed and permeabilized K562 cells gated on live single cells. Data acquired on the ZE5 Cell Analyzer.
Anti Carbonic Anhydrase VIII Antibody (PrecisionAb Monoclonal Antibody) gallery image 3
Figure A. StarBright Violet 670 conjugated Mouse anti Human HLA ABC (MCA81SBV670) and purified Mouse IgG2b isotype control (MCA691) detected with Goat anti Mouse IgG2b FITC (STAR134F). Figure B. StarBright Violet 670 conjugated Mouse anti Human HLA ABC (MCA81SBV670) and purified Carbonic Anhydrase VIII (VMA00350) detected with Goat anti Mouse IgG2b FITC (STAR134F). All experiments performed on Leucoperm (BUF09) fixed and permeabilized HEK293 cells gated on live single cells. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Carbonic Anhydrase VIII

Product Type
PrecisionAb Monoclonal
Isotype
IgG2b
Format
Purified
Specificity
Carbonic Anhydrase VIII

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Product Code Applications Pack Size List Price Your Price Qty
VMA00350
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Mouse anti Human carbonic anhydrase VIII antibody detects carbonic anhydrase VIII, also known as carbonic anhydrase-related protein. Carbonic anhydrase VIII is a member of a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa).

Mouse anti human carbonic anhydrase VIII antibody has been extensively validated for western blotting using whole cell lysates and detects a band of 33kDa. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti carbonic anhydrase VIII detects a band of approximately 33 kDa in K562 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant human carbonic anhydrase VIII
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml
List Price Your Price
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P35219
Entrez Gene
CA8
GO Terms
GO:0004089 carbonate dehydratase activity
GO:0006730 one-carbon metabolic process
GO:0008270 zinc ion binding

VMA00350

151215

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