CALPAIN-2 antibody | OTI3G1
KO Validated
IP-MS Validated
Mouse anti Human calpain-2 antibody recognizes calpain-2, also known as CANP 2, M-calpain, calcium-activated neutral proteinase 2, large [catalytic] subunit, calpain 2, large subunit, calpain M-type, calpain large polypeptide L2, calpain, large polypeptide L2, calpain-2 catalytic subunit and calpain-2 large subunit millimolar-calpain.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. The CAPN2 gene encodes calpain-2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for CAPN2 (provided by RefSeq, Mar 2009).
Mouse anti Human calpain-2 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. The CAPN2 gene encodes calpain-2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for CAPN2 (provided by RefSeq, Mar 2009).
Mouse anti Human calpain-2 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti calpain-2 detects a band of approximately 80 kDa in K562 cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human calpain-2 (NP_001739) produced in HEK293T cells
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P17655
- Entrez Gene
- CAPN2
- GO Terms
- GO:0005886 plasma membrane
- GO:0005737 cytoplasm
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of CALPAIN-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | |||
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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