CALPAIN-2 antibody | OTI3G1

Mouse anti CALPAIN-2

Product Type
PrecisionAb Monoclonal
Clone
OTI3G1
Isotype
IgG2a
Format
Purified
Specificity
CALPAIN-2

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VMA00164
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Mouse anti Human calpain-2 antibody recognizes calpain-2, also known as CANP 2, M-calpain, calcium-activated neutral proteinase 2, large [catalytic] subunit, calpain 2, large subunit, calpain M-type, calpain large polypeptide L2, calpain, large polypeptide L2, calpain-2 catalytic subunit and calpain-2 large subunit millimolar-calpain.

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. The CAPN2 gene encodes calpain-2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for CAPN2 (provided by RefSeq, Mar 2009).

Mouse anti Human calpain-2 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti calpain-2 detects a band of approximately 80 kDa in K562 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human calpain-2 (NP_001739) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P17655
Entrez Gene
CAPN2
GO Terms
GO:0005886 plasma membrane
GO:0005737 cytoplasm
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