CALPAIN-1 antibody

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Mouse anti CALPAIN-1

Mouse anti human calpain-1 antibody recognizes the calpain-1 catalytic subunit, also known as CAPN1, CANP 1, calcium-activated neutral proteinase 1, calpain mu-type, or micromolar-calpain. This antibody has been extensively validated for western blotting using whole cell lysates.

CALPAIN-1 Antibody with Control Lysate

Mouse anti human calpain-1 antibody recognizes the calpain-1 catalytic subunit, also known as CAPN1, CANP 1, calcium-activated neutral proteinase 1, calpain mu-type, calpain, large polypeptide L1, cell proliferation-inducing gene 30 protein or micromolar-calpain.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00353 WB 100 µl
VMA00353KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human calpain-1 antibody recognizes calpain-1, also known as CAPN1, calcium-activated neutral proteinase 1, calpain mu-type, calpain, large polypeptide L1, calpain-1 large subunit, cell proliferation-inducing gene 30 protein, cell proliferation-inducing protein 30 and micromolar-calpain.

The calpains, calcium-activated neutral proteases, are non-lysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. CAPN1 encodes the large subunit of the ubiquitous enzyme, calpain-1. Several transcript variants encoding two different isoforms have been found for CAPN1 (provided by RefSeq, Nov 2010).

Mouse anti Human calpain-1 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between amino acids from the human region of human calpain-1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P07384 Related reagents
Entrez Gene
CAPN1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005737 cytoplasm
GO:0008284 positive regulation of cell proliferation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of CALPAIN-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti calpain-1 detects a band of approximately 80 kDa in K562 cell lysates
Western Blotting
Anti calpain-1 detects a band of approximately 80 kDa in K562 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB