The calpains, calcium-activated neutral proteases, are non-lysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. CAPN1 encodes the large subunit of the ubiquitous enzyme, calpain-1. Several transcript variants encoding two different isoforms have been found for CAPN1 (provided by RefSeq, Nov 2010).
Mouse anti Human calpain-1 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti calpain-1 detects a band of approximately 80 kDa in K562 cell lysates
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- KLH conjugated synthetic peptide between amino acids from the human region of human calpain-1
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0005737 cytoplasm
- GO:0008284 positive regulation of cell proliferation
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of CALPAIN-1 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|