CALPAIN-1 antibody | 1376CT809.24.85

Mouse anti CALPAIN-1

Product Type
PrecisionAb Monoclonal
Clone
1376CT809.24.85
Isotype
IgG1
Format
Purified
Specificity
CALPAIN-1

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VMA00353
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Mouse anti Human calpain-1 antibody recognizes calpain-1, also known as CAPN1, calcium-activated neutral proteinase 1, calpain mu-type, calpain, large polypeptide L1, calpain-1 large subunit, cell proliferation-inducing gene 30 protein, cell proliferation-inducing protein 30 and micromolar-calpain.

The calpains, calcium-activated neutral proteases, are non-lysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. CAPN1 encodes the large subunit of the ubiquitous enzyme, calpain-1. Several transcript variants encoding two different isoforms have been found for CAPN1 (provided by RefSeq, Nov 2010).

Mouse anti Human calpain-1 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti calpain-1 detects a band of approximately 80 kDa in K562 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide between amino acids from the human region of human calpain-1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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UniProt
P07384
Entrez Gene
CAPN1
GO Terms
GO:0005886 plasma membrane
GO:0005737 cytoplasm
GO:0008284 positive regulation of cell proliferation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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