Calcitonin Receptor antibody | 30/7-9B4

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Mouse anti Human Calcitonin Receptor

Product Type
Monoclonal Antibody
Clone
30/7-9B4
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5749 E F IF P* WB 0.1 mg
Mouse anti Human Calcitonin Receptor antibody, clone 30/7-9B4 recognizes the human calcitonin receptor, a 508 amino acid multi pass transmembrane membrane glycoprotein with seven transmembrane domains, coupled to G protein messenger systems. The calcitonin receptor is expressed in kidney, central and peripheral nervous systems and on osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis. Mouse anti Human Calcitonin Receptor antibody, clone 30/7-9B4 recognizes an epitope within the extra-cellular domain, the epitope is common to both C1a and C1b human isoforms.

Calcitonin receptor expression is elevated in the inflammatory cells associated with diseased arteries and atherosclerotic plaques (Wookey et al. 2009) expression has also been noted within malignant cells of glioblastoma multiforme (Wookey et al. 2012).Several different isoforms of the calcitonin receptor, a result of alternative splicing and promotor usage have been identified. Mouse anti Human Calcitonin Receptor antibody, clone 30/7-9B4 was raised against the canonical isoform 1 but has been shown to bind to a truncated isoform lacking the N-terminal region of the receptor (Qi et al. 2012). The calcitonin receptor can associate with RAMP1, 2 and 3 to form discrete receptors for amylin.

Mouse anti human calcitonin receptor antibody, clone 30/7-9B4 has been successfully used for demonstrating calcitonin receptor expression on human cryostat tissue section by immunohistochemistry and western blotting (Wookey et al. 2012).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G-Sepharose/dialysed against PBS from tissue culture supernatant.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide derived from sequence situated on the extra-cellular domain of human calcitonin receptor.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P30988 Related reagents
Entrez Gene
CALCR Related reagents
GO Terms
GO:0005515 protein binding
GO:0004948 calcitonin receptor activity
GO:0005887 integral to plasma membrane
GO:0007189 activation of adenylate cyclase activity by G-protein signaling pathway
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0032841 calcitonin binding
GO:0045762 positive regulation of adenylate cyclase activity
GO:0051384 response to glucocorticoid stimulus
Regulatory
For research purposes only

Applications of Calcitonin Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Paraffin 1
Western Blotting
  1. 1PLP fixation is recommended for optimal results.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human kidney

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000B 150 Tests C P

Product Specific References

References for Calcitonin Receptor antibody

  1. Wookey, P.J. et al. (2012) The expression of calcitonin receptor detected in malignant cells of the brain tumour glioblastoma multiforme and functional properties in the cell line A172.
    Histopathology. 60 (6): 895-910.
  2. Qi, T. et al. (2013) Receptor activity-modifying protein-dependent impairment of calcitonin receptor splice variant Δ(1-47)hCT((a)) function
    Br J Pharmacol. 168: 644-57.
  3. Chen-An, P. et al. (2012) The Inhibitory Effect of Salmon Calcitonin on Tri-Iodothyronine Induction of Early Hypertrophy in Articular Cartilage
    PLoS One. 7(6): e40081.